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Comparative Bioavailability Study of Two Ramipril Tablet Formulations in Indonesian Healthy Volunteers

Yahdiana Harahap1, Lucy Sasongko2, Budi Prasaja3*, Windy Lusthom3, Evy C. Setiawan3, Raria S. Meliala3, Lipin3
1Department of Pharmacy, Faculty of Mathematic and Science, University of Indonesia, Depok (Indonesia)
2School of Pharmacy, Bandung Institute of Technology, Bandung (Indonesia)
3Clinisindo Laboratories, Jakarta (Indonesia)
*Corresponding author: Dr. Budi Prasaja, S.Si., Apt, MM,
Clinisindo Laboratories,
Jl. Ulujami Raya 12, Jakarta 12250 (Indonesia),
Tel        : +622173889918,
Fax      : +6221 73885453,
E-mail :budi.prasaja@clinisindo.com
Received November 05, 2009; Accepted December 13, 2009; Published December 13, 2009
Citation: Harahap Y, Sasongko L, Prasaja B, Lusthom W, Setiawan EC, et al. (2009) Comparative Bioavailability Study of Two Ramipril Tablet Formulations in Indonesian Healthy Volunteers. J Bioanal Biomed 1: 017-021. doi:10.4172/1948-593X.1000004
Copyright:© 2009 Harahap Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Aim
To compare the bioavailability of two ramipril tablet formulations– 10 mg Prohytens® tablets as test formulation and 10 mg Triatec® tablets as reference formulation.

Methods
A single-dosed, open-label, randomized two-way crossover design under fasting period with two weeks wash out period was evaluated in 24 subjects. For the analysis of pharmacokinetic properties, the blood samples were drawn taken up to 72 hours after dosing. Plasma concentration of ramipril and ramiprilat were determined using liquid chromatography – tandem mass spectrometry method with TurboIonSpray mode. Pharmacokinetic parameters AUC0-t, AUC0-∞ and Cmax were tested for bioequivalence after log-transformation of data and ratios of tmax were evaluated non-parametrically.

Results
The point estimates and 90% confidence intervals (CI) for AUC0-t, AUC0-∞ and Cmax for ramipril were 93.21% (85.67-101.41%), 93.45% (85.88-101.69%), 94.02% (80.09-110.38%) and for ramiprilat were 92.26% (87.76- 96.99%), 94.59% (89.71-99.73%) and 91.55% (84.88- 98.74%).

Conclusion
These results indicated that the two formulations of ramipril were bioequivalent and thus may be prescribed interchangeably.

 
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