Estimation of Swertiamarin in Enicostemma Littorale and Marketed Formulations Using HPLC-UV Method |
| Prawez Alam1, 2*, Mohammad Ali2, Rahul Singh3, Faiyaz Shakeel4 |
1Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudai Arabia |
| 2Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi-110062, India |
| 3Herbal Drug Research (Analytical Development) Ranbaxy Research laboratories, Gurgaon, Haryana, India |
| 4Department of Pharmaceutics, Faculty of Pharmacy, Al-Arab Medical University, Benghazi-5341, Libya |
| *Corresponding author: |
Dr. Prawez Alam,
Department of Pharmacognosy,
College of Pharmacy,
King Saud University, Riyadh, Saudi Arabia,
Tel : +966-532771723,
E-mail: prawez_pharma@yahoo.com |
|
| Received December 06, 2009; Accepted December 21, 2009; Published
December 21, 2009 |
Citation:Alam P, Ali M, Singh R, Shakeel F (2009) Estimation of
Swertiamarin in Enicostemma Littorale and Marketed Formulations Using
HPLC-UV Method. J Bioanal Biomed 1: 022-027. doi: 10.4172/1948-593X.1000005 |
Copyright:© 2009 Alam P, et al. This is an open-access article distributed
under the terms of the Creative Commons Attribution License,which
permits unrestricted use, distribution, and reproduction in any medium,
provided the original author and source are credited. |
| Abstract |
A simple, economic, reproducible, robust and precise
HPLC method for estimation of swertiamarin in both 60
% methanolic extract of Enicostemma littorale and marketed
formulations was developed and validated in present
investigation. The mobile phase composed of methanol
and water (90:10 % v/v), gave a sharp and well-defined
peak of swertiamarin at the retention time of 10.15 ± 1.52
min. The limit of detection (LOD) and limit of quantification
(LOQ) were 17.25 and 56.92 μg/ml respectively. The
proposed method with high degree of precision and accuracy
was employed for the estimation of swertiamarin in
methanolic extract and in marketed formulation.
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