The Bioavaliability of Hepatoprotective
Flavoniods in Hypericum Japonicum Extract |
| Ning Wang1, Yonggang Wang1, Peibo Li1, Wei Peng1, Tangning Xie1, Yibin Feng2, Weiwei Su1* |
| 1Guangzhou Quality R&D Center of Traditional Chinese Medicine, School of Life Science,
Sun Yat-sen University, Guangzhou, 510275, P.R. China |
2School of Chinese Medicine, The University of Hong Kong, 10 Sassoon Road, Pokfulam, Hong Kong |
| *Corresponding author: |
Dr. Weiwei Su,
Guangzhou Quality R&D Center
of Traditional Chinese Medicine,
School of Life Science, Sun Yat-sen
University,
Guangzhou, 510275, P.R. China,
E-mail: nwang@hku.hk |
|
| Received October 18, 2009; Accepted December 26, 2009; Published
December 26, 2009 |
Citation: Wang N, Wang Y, Li P, Peng W, Xie T, et al. (2009) The Bioavaliability of Hepatoprotective Flavoniods in Hypericum Japonicum Extract. J Bioanal Biomed 1: 033-038. doi:10.4172/1948-593X.1000007 |
Copyright: © 2009 Wang N, et al. This is an open-access article distributed
under the terms of the Creative Commons Attribution License, which
permits unrestricted use, distribution, and reproduction in any medium,
provided the original author and source are credited. |
| Abstract |
| Purpose: To study the absorption of main flavonoids in
Hypericum japonicum extract (HJE) with liver protective
property; Method: HPLC-ESI-MS was introduced to identify
and evaluate the flavonoids in HJE; Caco-2 cell monolayer
model was established and validated, and the compounds
in HJE, including quercetin (Q), quercetin-3-Orhamnoside
(Q-3-R), quercetin-7-O-rhamnoside (Q-7-R)
and quercetin-3-O-glucoside (Q-3-G) were administrated
in individual, paired or mixed form of the compounds to
the monolayer to evaluate their apparent permeability coefficients
(Papp value). The transport of HJE was also investigated,
mixture of pure components and HJE Inhibitor
was added to investigate the transport mechanism of
the compound mixture. The absorption of the four main
ingredients in HJE was then investigated in vivo. Result:
transportation of Q, Q-3-R Q-3-G but not Q-7-R trhough
Caco-2 monolayer was observed when they were administrated
individually. Increase of the transport of Q-3-G
and Q-7-R and decrease in Q were observed when the four
compounds were given in paired form; when the four flavonoids
were given as a whole (either in mixture of pure
compounds or in HJE), mass permeability of Q-3-R, Q-7-
R and Q-3-G was found. In vivo study identified the in
vitro investigation that the major active components of
HJE could be absorbed after orally administrated to mice. |
Conclusion: The increased transport of mixed active
components in HJE gives rise to the enhanced
hepatoprotetive effect of HJE, and therefore supports the
use of botanical drugs. |
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