Pharmacokinetic Evaluation of Metolazone
Tablets using healthy Human Volunteers |
Basvan Babu*, Selvadurai Muralidharan, Subramaniya Nainar Meyyanathan and
Bhojraj Suresh |
| Department of Pharmaceutical Analysis, J.S.S.College of Pharmacy, (Off Campus of JSS University, Mysore) Ootacamund, Rocklands, Ooty |
| *Corresponding author: |
Dr. Basvan Babu,
Department of Pharmaceutical
Analysis,
J.S.S.College of pharmacy, Tamilnadu, India,
Tel: +91-423-
2443393,
Fax: +91-423-2442937,
E-mail: thammababu@yahoo.co.in |
|
| Received November 25, 2009; Accepted December 22, 2009; Published
December 22, 2009 |
Citation: Babu B, Muralidharan S, Meyyanathan SN, Suresh B (2009) Pharmacokinetic Evaluation of Metolazone Tablets using healthy Human Volunteers. J Bioanal Biomed 1: 039-040. doi:10.4172/1948-593X.1000008 |
Copyright: © 2009 Babu B, et al. This is an open-access article distributed
under the terms of the Creative Commons Attribution License, which
permits unrestricted use, distribution, and reproduction in any medium,
provided the original author and source are credited. |
| Abstract |
| A sensitive and reproducible high performance liquid
chromatography (HPLC) method has been developed and
validated for the quantification of metolazone in human
plasma, after solid phase extraction (SPE). A Good resolution
was achieved on a reverse-phase LichroCART
Purospher® C18 column using the mobile phase acetonitrile–
0.5% triethylamine (35:65) in isocratic elution with
a total run time of 15 min. The analyte, metolazone, was
detected by using high performance liquid chromatography
with the support of photo diode array detector. Limit
of detection and Lower limit of quantification was found
to be 1 and 2.5 ng/mL. The present method was successfully
applied in the pharmacokinetic study of metolazone
in human plasma. |
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