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In-Vitro Release and Pharmacokinetics of Anti-tubercle Drug Ethionamide in Healthy Male Subjects

Mahmood Ahmad*, Asad Ullah Madni, Muhammad Usman
Department of Pharmacy, the Islamia University of Bahawalpur, Pakistan-63100
*Corresponding author: Dr.Mahmood Ahmad,
Department of Pharmacy,
the Islamia University of Bahawalpur,
Pakistan-63100,
E-mail: ma786_786@yahoo.com
Received December 26, 2009; Accepted December 30, 2009; Published December 30, 2009
Citation: Ahmad M, Madni AU, Usman M (2009) In-Vitro Release and Pharmacokinetics of Anti-tubercle Drug Ethionamide in Healthy Male Subjects. J Bioanal Biomed 1: 046-049. doi:10.4172/1948-593X.1000010
Copyright: © 2009 Ahmad M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
The aim of study was to assess the pharmacokinetics of ethionamide in the local population of healthy human subjects. Serum samples were taken from each of the selected subject at different time intervals. These samples were analyzed by using High Performance Liquid Chromatography consisting of reverse phase C18 column, UV detector set at 291nm. The mobile phase was consisted of 0.02 M disodium hydrogen phosphate and acetonitrile (75:25) and delivered at a rate of 1.5ml/min. The value of Cmax was found to be 1.941 ± 1.487 μg/ml (mean ± SEM) and Tmax was 1.75 ± 1.487 hours (mean ± SEM). The area under the curve (AUC) was 8.745 ± 0.536 (mean ± SEM). The elimination half life (t½) was found out as 1.995 ± 1.157 hours (mean ± SEM). The total body clearance (Cl) was determined as 32.591 ± 0.298 ml/hr/kg (mean ± SEM). It was concluded that ethionamide (Ethomid® Schazoo- Lahore, Pakistan) found in consistent with the values reported in the available literature. The study will be beneficial and valuable in designing dosage regimen for the patients on ethionamide therapy and can be utilized as guideline in accessing the bioavailability and pharmacokinetics parameters in clinical situations.
 
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