Non-Thermal Effects of Far-Infrared Ray (FIR) on Human
Hepatocellular Carcinoma Cells HepG2 and their Tumors |
Tatsuo Ishikawa1, Jun Ishibashi2, Kikuji Yamashita1*, Shine-Od Dalkhsuren1,
Kaori Sumida1, Takahumi Masui1 and Seiichiro Kitamura1 |
| 1Department of Oral and Maxillofacial Anatomy, Medical Science for Oral and Maxillofacial Regeneration,
Graduate School of Health Biosciences, University of Tokushima, 3-18-15 Kuramoto, Tokushima, 770-8504 Japan |
| 2Blanka Dental Office |
| *Corresponding author: |
Dr . Kikuji Yamashita, Department of Oral and
Maxillofacial Anatomy,
Medical Science for Oral and
Maxillofacial Regeneration,
Graduate School of Health Biosciences,
University of Tokushima, 3-18-15 Kuramoto,
Tokushima, 770-8504 Japan,
Tel : +81-88-6339120,
Fax : +81-88-6337320,
E-mail : kikuji@dent.tokushima-u.ac.jp |
|
| Received November 03, 2009; Accepted December 29, 2009; Published December 29, 2009 |
| Citation: Ishikawa T, Ishibashi J, Yamashita K, Dalkhsuren SO, Sumida
K, et al. (2009) Non-thermal Effects of Far-Infrared Ray (FIR) on Human
Hepatocellular Carcinoma Cells HepG2 and their Tumors. J Cancer Sci Ther 1: 078-082. doi:10.4172/1948-5956.1000012 |
| Copyright:© 2009 Ishikawa T, et al. This is an open-access article
distributed under the terms of the Creative Commons Attribution
License,which permits unrestricted use, distribution, and reproduction
in any medium, provided the original author and source are credited. |
| Abstract |
Background: We developed a cell culture CO2 incubator
and a mice rack that can continuously irradiate cells or
murine with FIR. Our goal is to make clear the non-thermal
effect of FIR on HepG2 with these instruments
morphologically. |
Methods: By using them, in vitro , we examined the
proliferation of cultured HepG2 cells with hematocytometer,
BrdU assay, WST-1 assay, HE staining, Toluidine blue
staining and microarray studies. And in vivo, we measured
the tumors, observed the sections by IHC, DAPI staining
with light microscopes and performed microarray studies. |
Results: Proliferation of HepG2 cells were suppressed
(e.g., cell count declined by 34% after 10 days of FIR
irradiation), tumor volumes reduced by 86% after 30 days
of FIR irradiation, mRNA of Vascular Endothelial Growth
Factor (VEGF) decreased by 48%, vascular area in cross
sections from the tumors decreased 60% compared with
the control. More frequent properties in apoptosis were
observed by TUNEL and DAPI staining in FIR-treated
groups. Body weight of mice increased compared with the
control. Oxydation and Reduction (Redox) reactions by
H+ (proton and electron)/O2- (a kind of Reactive Oxygen
Species (ROS)) were induced by FIR. |
Conclusions: These results clarified that FIR inhibited
the proliferation of HepG2 at non-thermal circumstances
(at 25±0.5, 37±0.5°C). FIR will serve as a tool against
diseases induced by HepG2.
|
|
|
|