Differential Display Analysis of cDNA Involved in Microbial
Mats Response after Heavy Fuel Oil Contamination |
| Sylvain Bordenave, Marisol Goni-Urriza, Pierre Caumette, Robert Duran* |
| Université de Pau et des Pays de l’Adour, Institut Pluridisciplinaire de Recherche Environnement et Matériaux – Equipe Environnement et
Microbiologie, UMR CNRS 5254 (IPREM - EEM), IBEAS - UFR Sciences et Techniques, BP 1155 F64013 Pau cedex France |
| *Corresponding author: |
Dr. Robert Duran,Université de Pau et des Pays
de l’Adour,
Institut Pluridisciplinaire de Recherche Environnement et
Matériaux - Equipe Environnement et Microbiologie, UMR CNRS 5254
(IPREM - EEM),
IBEAS - UFR Sciences et Techniques,
BP 1155 F64013
Pau cedex France,
Tel : (33) 5 59407468,
Fax : (33) 5 59407494,
E-mail : robert.duran@univ-pau.fr |
|
| Received December 20, 2009; Accepted December 28, 2009; Published December 28, 2009 |
| Citation: Bordenave S, Goni-Urriza M, Caumette P, Duran R (2009) Differential Display Analysis of cDNA Involved in Microbial Mats Response
after Heavy Fuel Oil Contamination. J Microbial Biochem Technol 1:
001-004. doi:10.4172/1948-5948.1000001 |
| Copyright: ©2009 Bordenave S, et al. This is an open-access article
distributed under the terms of the Creative Commons Attribution
License,which permits unrestricted use, distribution, and reproduction
in any medium, provided the original author and source are credited. |
| Abstract |
Pristine microbial mats from Camargue salterns (France)
maintained in microcosm were contaminated by Erika fuel
oil in order to identify gene sequences induced in response
to heavy fuel oil contamination. The differential display
approach was adapted to detect differentially expressed
mRNA in complex bacterial communities. Among the six
differentially expressed (DD) cDNA fragments isolated,
one was identified and associated with an ABC-type efflux
pump. A second DD-fragment was related to a conserved
hypothetical protein found in many different bacterial species.
Despite differentially expressed fragments could not
be clearly identified, this study reveals new perspectives
for the improvement of our knowledge on the response of
microbial community after petroleum contamination. |
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