The Effects of NO and AgNO3 on Cell Growth and
Salidroside Synthesis in Rhodiola Sachalinensis
A.Bor. Cell Suspension Culture |
AI Jiang-ning, Zhou Bin, JIA Jing-ming* |
| Department of Traditional Chinese Medicines, Shenyang Pharmaceutical University,103 Wenhua Road, Shenyang, 110016, China |
| *Corresponding author: |
Dr. JIA Jing-ming,
Department of Traditional Chinese Medicines,
Shenyang Pharmaceutical University,103 Wenhua Road,
Shenyang, 110016, China |
|
| Received April 13, 2009; Accepted November 10, 2009; Published November 10, 2009 |
| Citation: Jiang-ning AI, Bin Z, Jing-ming JIA(2009) The Effects of NO
and AgNO3 on Cell Growth and Salidroside Synthesis in Rhodiola
sachalinensis A.Bor. Cell Suspension Culture. J Microbial Biochem
Technol 1: 011-014. doi:10.4172/1948-5948.1000003 |
| Copyright:© 2009 Jiang-ning AI, et al. This is an open-access article
distributed under the terms of the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any
medium, provided the original author and source are credited. |
| Abstract |
| The purpose of this work was to investigate the effect of
abiotic elicitors on the production of salidroside in Rhodiola sachalinensis A.Bor. Different concentration of
each elicitor was respectively added into the cell suspension
culture in different periods of cell culture. The content
of salidroside was determined by high performance
liquid chromatography (HPLC). NO could enhance cell
growth and the synthesis of salidroside, whereas AgNO3 inhibited cell growth, and promoted the synthesis of
salidroside. 50 μmol/L of SNP as the donator of NO and
60 μmol/L of AgNO3 were added into cell suspension culture
on the 12th day. And the contents of salidroside were
significantly increased up to 2.2 fold and 2.0 fold respectively.
Therefore the elicitation by NO and AgNO3 can
effectively promote accumulation of the secondary metabolite
in plant cell culture. |
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