Involvement of Serine Threonine Protein Kinase,
PknL, from Mycobacterium Tuberculosis H37Rv
in Starvation Response of Mycobacteria |
| Harini Lakshminarayan1, A. Rajaram2 and Sujatha Narayanan1* |
| 1Tuberculosis Research Centre, Mayor V R Ramanathan Road, Chetpet, Chennai-600 031 |
| 2Central Leather Research Institute, Adayar, Chennai -600020 |
| *Corresponding author: |
Dr. Sujatha Narayanan,
Scientist F Tuberculosis
Research Centre (ICMR),
Mayor V R Ramanathan Road Chetpet,
Chennai-600 031 Tamil Nadu, India,
Tel : 91-44-28369627,
Fax : 91-44-28362528,
E-mail : sujatha.sujatha36@gmail.com |
|
| Received December 21, 2009; Accepted December 27, 2009; Published December 27, 2009 |
Citation: Lakshminarayan H, Rajaram A, Narayanan S (2009) Involvement of Serine Threonine Protein Kinase, PknL, from Mycobacterium Tuberculosis H37Rv in Starvation Response of Mycobacteria. J Microbial Biochem Technol 1: 030-036. doi:10.4172/1948-5948.1000006 |
Copyright: © 2009 Lakshminarayan H, et al. This is an open-access article
distributed under the terms of the Creative Commons Attribution
License,which permits unrestricted use, distribution, and reproduction
in any medium, provided the original author and source are credited. |
| Abstract |
The adaptation to nutrient depletion in bacteria involves
a highly organized series of intracellular events that enable
them to adapt to starvation conditions. The regulatory
effect of serine threonine protein kinase, PknL, fromMycobacterium tuberculosis strain H37Rv was investigated
under nutrient deprived conditions that simulate circumstances
leading to latency. Recombinant PknL was expressed
in Mycobacterium smegmatis strain mc2 155 in its
wild type and mutant forms. In vitro growth kinetics experiments
revealed that clone expressing active PknL had
a significant growth advantage under nutrient limiting
conditions. Experiments were conducted to ascertain thein silico predictions of the involvement of PknL in regulating
glutamine metabolism in mycobacteria. Furthermore,
a role for PknL in cell wall biogenesis/cell division
was shown by scanning electron microscopy. |
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