Optimization of Submerged Culture
Conditions for Exo-Polysaccharides Production
by Streptomyces Nasri-UV 135 in Bioreactor |
| Usama Beshay1*, Ayman Daba2, Yousry Gohar3 |
| 1Mubarak City for Scientific Research and Technology Applications, Genetic Engineering and Biotechnology
Research Institute (GEBRI), Bioprocess Development Dept, New Bourg El-Arab City, 21934 Alexandria, Egypt |
| 2Mubarak City for Scientific Research and Technology Applications, Genetic Engineering and Biotechnology
Research Institute (GEBRI), Pharmaceuticals Bioproducts Dept, New Bourg El-Arab City, 21934 Alexandria, Egypt |
3Alexandria University, Faculty of Science, Division of Microbiology, Alexandria, Egypt |
| *Corresponding author: |
Dr. Usama Beshay,
Bioprocess Development
Dept. Genetic Engineering & Biotechnology Research Institute (GEBRI)
Mubarak City for Scientific Research and Technology Applications
(MUCSAT) New Bourg El-Arab City, Universities and Research District,
21934 Alexandria, Egypt,
Fax : +2034593423
E-mail : ugabra10@hotmail.com, u.beshay@mucsat.sci.eg |
|
| Received December 19, 2009; Accepted December 27, 2009; Published December 28, 2009 |
Citation: Beshay U, Daba A, Gohar Y(2009) Optimization of submerged culture conditions for exo-polysaccharides production by Streptomyces nasri-UV 135 in bioreactor. J Microbial Biochem Technol 1: 043-046. doi:10.4172/1948-5948.1000008 |
Copyright: © 2009 Beshay U, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License,which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Abstract |
| Production of Exopolysaccharides “EPS” and microbial
biomass by Streptomyces nasri were influenced by the type
of carbon source (glycerol, xylose, fructose, galactose,
glucose, mannose, sucrose, lactose, maltose, dextrin,
soluble starch, corn starch and potato starch) and the
nitrogen source (glycine, aspartic acid, glutamic acid,
proline, ammonium sulphate, sodium nitrate and beef
extract ) used in the medium. |
Xylose and glycine were the most suitable sources of
carbon and nitrogen, respectively, for both production of
EPS and mycelial growth. The highest EPS production
was obtained in a medium containing (g/l) 30 xylose, 2.7
glycine, 4.0 NaCl, 0.5 MgSO4, 1.0 K2HPO4, and 1.0
CaCO3. Exopolysaccharides production and mycelial
growth in the above suggested medium were significantly
increased in a 3-l stirred tank bioreactor, where the maximum
EPS concentration was 8.73 g/l, which was an approximately
1.6 time higher than that in shake culture. |
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