Primer Designing for Dreb1A, A Cold Induced Gene |
Neha Garg1,3, Sachin Pundhir2, Anil Prakash3 and Anil Kumar2* |
| 1Division of Plant Physiology, Institute of Biochemistry & Biology, University of Potsdam, Karl-Liebknecht-STR 24-25, Haus 20 D-14476, Golm, Germany |
| 2School of Biotechnology, Devi Ahilya University, Khandwa Rd., Indore-452001, India |
| 3Biotechnology Department, Barkatullah University, Bhopal-462026, India |
| *Corresponding author: |
Dr. Anil Kumar, Professor & Head, School of Biotechnology, Devi Ahilya University, Khandwa Road
Campus, INDORE-452001, INDIA,
Phone:91-731-2470372, 2470373,
Fax:91-731-2470372,
E-mail: ak_sbt@yahoo.com |
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| Received Feb 02, 2008; Accepted April 15, 2008; Published April 22, 2008 |
| Citation: Neha G, Sachin P, Anil P, Anil K (2008) Primer Designing for Dreb1A, A Cold Induced Gene . J Proteomics Bioinform 1: 028-035. doi:10.4172/jpb.1000006 |
| Copyright:©2008 Neha G, etal. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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| Abstract |
Primer designing for cold induced gene, DREB1A is done using Primer3 software. Seventeen sequences related to DREB1A gene
were retrieved from UniGene, GenBank and RefSeq databases using Entrez. On multiple sequence alignment, a 286 bp conserved
region was identified. Potential primers for the conserved region and for seventeen nucleotide sequences were determined using
Primer3. The properties of these potential primers were analyzed using Premier Biosoft’s NetPrimer tool. One forward (5’ end) and
one backward (3’ end) primer having 50 to 60% GC content, 52 to 58oC Tm and absence of secondary structures were finalized.
Specificity of the primers was validated by carrying out local alignment against the NCBI’s nr database through BLAST. All the
alignments showed significant alignment to DREB1A gene validating the specificity of the primers. |
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