Proteases occur naturally in all organisms. These enzymes are involved in a multitude of physiological reactions from simple digestion of food proteins to regulate a great variety of physiological processes including processing and molecular assembly of nascent polypeptide chain, processing of protein hormone and enzyme precursors to the development and fertilization. Deregulation of proteolytic enzymes leads to human pathologies including arthritis, stroke , dementia , etc. Four mechanistic classes of enzymes have been identified. They are serine proteinase, cysteine proteinase, aspartic proteinase and metallo-proteinase. There is growing literature on proteolytic enzymes. This paper contains updated bibliography of Proteolytic Enzyme and Physicochemical properties of Proteolytic Enzyme including, enzyme’s class, source, EC, molecular weight, N-terminal, C-terminal ,thiols, activators, inhibitors, bond specificity and comments. This database will be of high values for researchers and students working in this area.