SELDI Based Proteomic Determination of Hepatic Biomarkers in Mouse Serum Following Acetaminophen Administration |
| Huixiao Hong1*, Candee Teitel2, Laura P. James3, Weida Tong1, Jack A. Hinson3, James C. Fuscoe1, Yvonne Dragan1*¶ |
1Division of Systems Toxicology, National Center for Toxicological Research,
Food and Drug Administration, Jefferson, AR 72079, USA |
| 2Division of Personalized Nutrition and Medicine, National Center for Toxicological Research,
Food and Drug Administration, Jefferson, AR 72079, USA |
| 3Departments of Pediatrics and Pharmacology and Toxicology, University of Arkansas for
Medical Sciences, Little Rock, AR 72205, USA |
| *Corresponding author: |
Dr. Huixiao Hong, Division of Systems Toxicology,
National Center for Toxicological Research,
Food and Drug Administration, Jefferson, AR 72079, USA,
Phone: 870-543-7296;
E-mail: Huixiao.Hong@fda.hhs.gov or
Dr. Yvonne Dragan, AstraZeneca Pharmaceuticals,
1800 Concord Pike CDRL-138,
Wilmington, DE 19803, USA,
Phone: 302-885-3186;
E-mail: Yvonne.Dragan@astrazeneca.com |
|
| ¶Current Address: AstraZeneca Pharmaceuticals, 1800 Concord Pike CDRL-138, Wilmington, DE 19803, USA |
| The views presented in this article do not necessarily reflect those of the US Food and Drug Administration |
| Received November 02, 2008; Accepted November 17, 2008; Published November 18, 2008 |
| Citation:Huixiao H, Candee T, Laura PJ, Weida T, Jack AH, eta l. (2008) SELDI Based Proteomic Determination of Hepatic
Biomarkers in Mouse Serum Following Acetaminophen Administration. J Proteomics Bioinform 1: 424-436. doi:10.4172/jpb.1000051 |
| Copyright: © 2008 Huixiao H, etal. This is an open-access article distributed under the terms of the Creative Commons
Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author
and source are credited. |
| Abstract |
Drug induced toxicities account for nearly half of the cases of acute liver failure in adults over age 50 in the US. Acetaminophen (APAP) is a commonly used non-prescription drug that can be purchased in drug stores and supermarkets. Cases of unintentional APAP overdose and associated hepatotoxicity have been reported to the FDA. Early detection of hepatotoxicity caused by APAP is of great interest to both the scientific and public communities. Therefore, in this study SELDI based analysis of mouse serum following APAP administration was conducted to demonstrate the feasibility of identifying hepatic toxicity biomarkers from a readily available body fluid. Late hepatotoxicity caused by APAP was detected by SELDI. Furthermore, early stage hepatotoxicity caused by APAP that was not detected pathologically was clearly discernable using SELDI. Our results suggest that SELDI analysis can be used for early detection of hepatotoxicity based on the expression pattern of the biomarker peaks. |
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