Selaginella bryopteris (L.) Bak is a resurrection plant. Its detached fronds have unique ability to survive desiccation similar to that of whole plant. In order to understand the mechanisms of desiccation tolerance, proteome studies were carried out using fronds of the Selaginella bryopteris to reveal proteins that were differentially expressed in response to dehydration and rehydration. There was not much difference in electrolyte leakage between control, dehydrated and rehydrated fronds. During dehydration the plants showed only respiration and drop in F_v/F_m values. Both fluorescence and photosynthesis regained totally after rehydration. About 250 protein spots were reproducibly detected and analyzed. Analysis of the identi?ed proteins revealed that proteins involved in protein destination and degradation were more expressed in desiccated fronds. These findings tentatively indicate that some of the proteins could contribute a physiological advantage to S. bryopteris under desiccation.