| Research Article |
Open Access |
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| Proteome Profile of Zebrafish Brain Based on Gel LC-ESI MS/MS Analysis |
| Sachin Kumar Singh1, Komarla Setty Rakesh2, Kalidoss Ramamoorthy3, Attluri V Pardha Saradhi4, Mohammed Mohammed Idris5* |
| 1Research Fellow, Department of Neurobiology, W309, CCMB, Uppal Road, Hyderabad 500007 |
| 2Senior Research fellow, Proteomics Department, CCMB, Uppal Road, Hyderabad 500007 |
| 3Research Fellow, Proteomics Department, CCMB, Uppal Road, Hyderabad 500007 |
| 4Research fellow Department of Neurobiology, W309, CCMB, Uppal Road, Hyderabad 500007 |
| 5Scientist, Department of Neurobiology, W309, CCMB, Uppal Road, Hydrabad 500007 |
| *Corresponding author: |
Mohammed Mohammed Idris,
Scientist, Department of
Neurobiology, W309, CCMB,
Uppal Road, Hyderabad 500007,
E-mail: idris@ccmb.res.in |
|
| Received March 03, 2010; Accepted April 19, 2010; Published April 19, 2010 |
| Citation: Singh SK, Rakesh KS, Ramamoorthy K, Pardha Saradhi AV, Idris MM
(2010) Proteome Profile of Zebrafish Brain Based on Gel LC-ESI MS/MS Analysis.
J Proteomics Bioinform 3: 135-142. doi: 10.4172/jpb.1000132 |
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| Copyright: © 2010 Singh SK, et al. This is an open-access article distributed under
the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original author and
source are credited. |
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| Abstract |
| The zebrafish (Danio rerio) is the extensively used alternate vertebrate model animal for understanding the brain function, development and evolution. Detailed brain proteome map of zebrafish is still not known inspite of its broad usage in developmental and neurological studies. We present here the large scale proteome profile of the zebrafish brain at the normal condition based on gel LC ESI MS/MS analysis. A total of 8475 different proteins details were identified based on this study with less than 1% false positive rate. All the proteins details obtained from this study were duly submitted to the database for validation and obtained accession numbers. The various proteins identified in this study were found to be involved in different biological activities, neurological functions and network pathways. With the availability of genomics information, this extensive study of proteomic profile of zebrafish brain tissue provided a complete view and details about the various proteins expressed in brain at the basal state. This study can lead to understand various new biomarkers underlying for various biological characteristics like development and neurological disease. |
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