Journal of Analytical & Bioanalytical Techniques
Open Access

Research Article

Chemometrical Evaluation of Metoprolol Tartarate Enantiomers Separation Applying Conventional Achiral Chromatography

Ivkovic B^1*, Karljikovic-Rajic K², Vujic Z¹ and Ibric S³

¹Department of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmacy, University of Belgrade, Serbia

²Department of Analytical Chemistry, Faculty of Pharmacy, University of Belgrade, Serbia

³Department of Pharmaceutical Technology and Cosmetology, University of Belgrade, Serbia

*Corresponding Author:

Branka Ivkovic
Department of Pharmaceutical Chemistry and Drug Analysis
Faculty of Pharmacy, University of Belgrade
Vojvode Stepe 450, 11221 Belgrade-Kumodraz
PO Box 146, Serbia
Tel:+381113951335
Fax: +381113972840
E-mail: blucic@pharmacy.bg.ac.rs

Received Date: January 27, 2016; Accepted Date: February 24, 2016; Published Date: March 04, 2016

Citation: Ivkovic B, Karljikovic-Rajic K, Vujic Z, Ibric S (2016) Chemometrical Evaluation of Metoprolol Tartarate Enantiomers Separation Applying Conventional Achiral Chromatography. J Anal Bioanal Tech 7:303. doi:10.4172/2155-9872.1000303

Copyright: © 2016 Ivkovic B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

In this paper, the separation of metoprolol tartarate enantiomers using conventional achiral chromatography was followed employing experimental design 24. Design was useful tool in evaluation of chromatographic behavior of enantiomers. For the separation, Supelcosil LC18 4.6 mm × 250 mm, 5 m particle size column was used. UV detection was performed at 275 nm. Because of the large number of factors which can influence chromatographic separation, in preliminary study ″one factor at a time″ method was applied to define values of important factors. As outputs, retention factor, selectivity factor and resolution factors were chosen. Chromatographic behavior of investigated enantiomers was affected by acetonitrile content, chiral modificator content in the mobile phase and flow rate the most, demonstrated by obtained linear models. On the basis of results from screening of experiment, factors with strong influence on the separation, were analyzed in method optimization applying response surface methodology (RSM). The appropriate region of chromatographic behavior of metoprolol tartarate enantiomers was defined using three-D graphs and analysis of variance. The methodology proposed represents an efficient approach in resolving the problem of searching for optimal HPLC chromatographic conditions via experimental design.

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