Figure 5: The EpCAM-specific #1-F aptamer preferentially distinguishes epithelial cancer cells in cell mixtures. EpCAM-expressing MDAMB- 468 cells were mixed with U937 control cells (a) or whole blood (b), stained with the #1-F aptamer and the anti-EpCAM antibody. Resultant cell binding was examined by flow cytometry. (c) MDA-MB-468 cells were mixed with the nucleated white blood cells, and cell mixture was stained with the anti-EpCAM antibody and the #1-F aptamer. Resultant cell binding was analyzed by flow cytometry. (Note: P1: gated living cells; P2: gated EpCAM-positive cells identified by EpCAM antibody)