Under-expression of miR-100 may be a new Carcinogenic pathway for low-grade pTa Bladder Urothelial Carcinomas

Mar 5, 2012 by Journal of Molecular Biomarkers and Diagnosis

Objectives: The pathways involved in the carcinogenesis of bladder urothelial carcinoma have been well
established and are used for the development of new diagnostic and prognostic markers for the disease. The
main genetic pathway for the development of low-grade pTa urothelial carcinomas is related to FGFR3 mutation.
MicroRNAs have been related to processes involved in carcinogenesis in many organs, and miR-100 was recently
shown to target FGFR3 messenger RNA. Our aim was to study the profile of expression of miR-100 and FGFR3 in
low-grade, pTa bladder urothelial carcinoma.
Methods and Materials: Using qRT-PCR, we studied the expression of miR-100 and FGFR3 in 30 patients who
had undergone transurethral resection of low-grade, pTa bladder urothelial carcinoma.
Results and Conclusion: There was under-expression of miR-100 and over-expression of FGFR3 in 100% of
the specimens. Under-expression of miR-100 might be an alternative pathway for low-grade pTa urothelial bladder
carcinogenesis and the identification of this molecular alteration may constitute a new diagnostic and prognostic
marker for the disease.