Mass Spectrometry in Proteome Research

Proteomics is an emerging field that has been highly enabled by the human genome project. Proteins are the products of genes, the machinery of the cells in our bodies. Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins. Mass spectrometry is an important emerging method for the characterization of proteins. Two-dimensional gel electrophoresis can be used to create cellular protein maps which give a quantitative and qualitative picture of the proteome. Mass spectrometry is the method of choice for the rapid large-scale identification of these proteomes and their modifications. Proteogenomics uses mass spectrometry data to experimentally validate gene products and to assist in the process of genome annotation and comparison. There are almost 3000 people from 60-65 universities in USA working for Proteomics. Research Awards in the amount of $35,000 each are presented annually. Two awards are fully sponsored by Thermo Scientific and Waters Corporation.

Proteins are the products of genes, the machinery of the cells in our bodies. Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins. Mass spectrometry is an important emerging method for the characterization of proteins. Two-dimensional gel electrophoresis can be used to create cellular protein maps which give a quantitative and qualitative picture of the proteome. Mass spectrometry is the method of choice for the rapid large-scale identification of these proteomes and their modifications. Proteogenomics uses mass spectrometry data to experimentally validate gene products and to assist in the process of genome annotation and comparison.

  • Matrix-Assisted laser desorption (MALDI-TOF-MS)
  • Electrospray ionization mass spectrometry (ESI-MS)
  • Liquid chromatography mass spectrometry (LC-MS)
  • Multidimensional protein identification technology
  • Protein identification and validation

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