ISSN : 2153-2435
Pharmaceutica Analytica Acta
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Determination of Cholesterol and its Oxides

Hideharu Shintani*
Chuo University,School of Science,1-13-27,Kasuga Bunkyo 112-0003 Tokyo,Japan
Corresponding Author : Hideharu Shintani
Chuo University, School of Science
1-13-27, Kasuga Bunkyo 112-0003 Tokyo, Japan
Tel: +81425922336
Received June 09, 2013; Accepted June 15, 2013; Published June 19, 2013
Citation:Shintani H (2013) Determination of Cholesterol and its Oxides. Pharm Anal Acta S1:003. doi:10.4172/2153-2435.S1-003
Copyright: © 2013 Shintani H. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at
DownloadPubmed DownloadScholar Google

Visit for more related articles at Pharmaceutica Analytica Acta



Cholesterol oxidation products; Atherosclerosis; HPLCMS; GLC-MS
Several cholesterol oxidation products might play important roles in the development of atherosclerosis [1]. This concept is fostered by mounting interest in the role of oxidatively modified lipoproteins and cholesterol in atherogenesis. Cholesterol oxides are widely encountered in foods and biological tissues. Here the analysis of plasma cholesterol oxidation products by high-performance liquid chromatography-mass spectrometry (HPLC-MS) [2,3] is described
1. Bllod is drawn into 10-mL Vacutainer tubes containing EDTA (15% w/v, 0.10 mL).
2. Lipids are extracted from plasma (1.0-mL) in 12-mL borosilicate screw cap tubes by addition of chloroform-methanol (2:1, v/v 6.0 mL) containing BHT (0.01% w/v).
3. Internal standard stock solution (5α-cholestane in toluene, 1.0 mg mL-1, 100 μL) is added to each sample and the tubes are sealed under argon and mixed in a rotary drum for 30 min at room temperature.
4. After centrifugation at 3,000 rpm for 15 min, the organic phase is isolated and the aqueous phase is re-extracted with chloroform-methanol (3.0 mL).
5. The pooled organic phases are evaporated to dryness under nitrogen and the residue, dissolved in ethanol or hexaneisopropanol (1:1, v/v), is analysed by HPLC-MS. (HPLCMS analysis was performed by means of a Hewlett-Packard HPLC 1090A chromatograph, a particle beam interface, and a Hewlett-Packard 5988A mass spectrometer. Compounds were separated on an Altex-Ultrasphere-Si 250 column with 96:4 (v/v) hexane-isopropanol as mobile phase at a flow rate of 0.4 mL min-1).
The principal ions monitored for analysis of cholesterol oxides are listed in Table 1.
Together with HPLC-MS, capillary gas chromatography-mass spectrometry (GC-MS) can also be used for the analysis of cholesterol oxides after derivatization, usually to the trimethylsilyl ethers.

Select your language of interest to view the total content in your interested language
Share This Article
Relevant Topics
Disc Advances in pharmaceutical analytical methods
Disc Air analysis
Disc Analytical Biochemistry Techniques
Disc Analytical Chemistry
Disc Analytical Chromatography
Disc Analytical Techniques
Disc Antigen – Antibody Reaction
Disc Bioanalysis
Disc Bioanalytical Techniques
Disc Biomedical Chromatography
Disc Capillary Electrochromatography
Disc Capillary Electrophoresis
Disc Chemical analysis
Disc Chemometric Analysis
Disc Chromatography
Disc Competitive analysis of drugs
Disc Differential Scanning Calorimetry
Disc Drug Delivery using Nanotechnology
Disc Drug Designing
Disc Drug Resistance
Disc Drug analysis
Disc Electrophoresis
Disc Extraction Chromatography
Disc Filtration
Disc Flow Injection Analysis
Disc Fragment Based Drug Design
Disc GC-MS
Disc Gravimetric Analysis
Disc Heat Shock Proteins
Disc Heterocyclic Compounds
Disc Immuno Affinity Chromatography
Disc In Silico Methods
Disc Infrared Spectroscopy
Disc Ion-exchange chromatography
Disc LC-MS
Disc LC-MS principles
Disc Method Validation
Disc Molecular Modeling
Disc Nanomedicals
Disc Peptidomimetic
Disc Pharmaceutical Analytical Techniques
Disc Pharmaceutical Sciences
Disc Pharmaceutical analysis
Disc Pharmaceutical analytical market
Disc Pharmaceutical formulation
Disc Pharmaceutical technology
Disc Pharmaceutics
Disc Product analysis and marketing
Disc Protease Substrate/Inhibitor
Disc Qualitative Analysis
Disc Quality assurance in pharmaceutical analysis
Disc Receptor Agonist/ Antagonist
Disc Separation Techniques
Disc Spectrometry
Disc Spectrophotometry
Disc Spectroscopy
Disc Structure Based Drug Design
Disc Super Critical Fluid Chromatography
Disc Synthetic Organic Chemistry
Disc Validation
Disc screening and Drug Design
Recommended Journals
Disc Journal of Diagnostic Techniques and Biomedical Analysis
Disc Journal of Pharmaceutical Analysis
Disc Pharmaceutical Analytical Chemistry: Open Access
Disc Journal of Chromatography & Separation Techniques
Disc Journal of Analytical & Bioanalytical Techniques
Disc Pharmaceutics Research Journal
Disc Drug Designing Journal
  View More»
Recommended Conferences
Disc World Congress on Chromatography
September 21-23, 2016 Amsterdam, Netherlands
Disc World Chromatography Congress
September 29-Oct 1, 2016, Miami, USA
Disc 3rd Asia Pacific Mass Spectrometry Congress
October 10-12, 2016 Kuala Lumpur, Malaysia
Disc 2nd Conference on Parenterals
October 24-26, 2016 Istanbul, Turkey
Disc World Pharma Congress
Nov 07-Nov 09, 2016 Las Vegas, USA
Disc International Pharmaceutical Method Development and Validation Conference
November 24-25, 2016 Dubai, UAE
Article Tools
Disc Export citation
Disc Share/Blog this article
Article usage
  Total views: 11140
  [From(publication date):
specialissue-2013 - May 31, 2016]
  Breakdown by view type
  HTML page views : 7411
  PDF downloads :3729

Post your comment

captcha   Reload  Can't read the image? click here to refresh

OMICS International Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
OMICS International Conferences 2016-17
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2016 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version