ISSN : 2153-2435
Pharmaceutica Analytica Acta
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Is Supplemental-Antioxidant Helpful to Human Health?

Hideharu Shintani*
Chuo University, School of Science, 1-13-27, Kasuga Bunkyo 112-0003 Tokyo, Japan
Corresponding Author : Hideharu Shintani
Chuo University, School of Science
1-13-27, Kasuga Bunkyo 112-0003 Tokyo, Japan
Tel: +81425922336
E-mail: shintani@mail.hinocatv.ne.jp
Received June 09, 2013; Accepted June 13, 2013; Published June 15, 2013
Citation: Shintani H (2013) Pharmaceutical Sciences: Analytical Methods.Pharmaceut Anal Acta 4:e151.doi:10.4172/2153-2435.1000e151
Copyright: © 2013 Shintani H. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Analytical methods used in pharmaceutical science must be sufficiently accurate, specific, sensitive, selective and precise to conform to the regulatory requirements as set out in the relevant guidelines of “The International Conference of Technical Requirements for the Registration of Pharmaceutical for Human Use” (ICH). It must recognize that the analysis conditions of pure sample and that of crude sample significantly differ. The analysis of the former is quite easy, but the latter conditions are quite tough and furthermore so often we must analysis sample in crude status, for example sample in blood or body fluid. For example in case of HPLC analysis of crude samples, the pretreatment procedure requires significantly. Several methodologies for pretreatment such as solid phase extraction (SPE), Ultrafiltration, dialysis and so on are reported and commercially available. The editor compared these methods and one method is found to be recommendable. But this recommendation is restricted to hydrophilic compounds in body fluid, so if target differs, the result will differ. In that sense, readers themselves must validate which is most appropriate for your purpose.
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