Oladele and Oluwole Olakunle

"> Oladele and Oluwole Olakunle"/>
Home   |  Publications   |   Conferences    |  Join   |   Contact   | Sitemap  

Journal of Plant Pathology & Microbiology

Open Access
ISSN: 2157-7471
home » microorganisms-associated-with-the-deterioration-of-fresh-leafy-indian-spinach-in-storage-2157-7471 Rss Feed Rss Feed
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 1000+ Global Events inclusive of 300+ Conferences, 500+ Upcoming and Previous Symposiums and
Workshops on Pharma, Medicine, Science and Technology
Research Article Open Access
Microorganisms Associated with the Deterioration of Fresh Leafy Indian Spinach in Storage
Oladele* and Oluwole Olakunle
Department of Biology Federal University of Technology, P.M.B 704, Akure, Nigeria
Corresponding Author : Dr. Oladele
Department of Biology Federal University of Technology
P.M.B 704, Akure, Nigeria
E-mail: richcometh2003@yahoo.com
Received September 02, 2011; Accepted October 24, 2011; Published October 26, 2011
Citation: Oladele, Olakunle O (2011) Microorganisms Associated with the Deterioration of Fresh Leafy Indian Spinach in Storage. J Plant Pathol Microbiol 2:110.doi: 10.4172/2157-7471.1000110
Copyright: © 2011 Oladele, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at
DownloadPubmed DownloadScholar Google
Visit for more related articles at
DownloadJournal of Plant Pathology & Microbiology

This work investigated microorganisms associated with the deterioration of fresh leafy Indian spinach. Microbial counts involve the use of pour plate method (PPM). The deterioration, marked by loss of green colour to mushiness of the spinach leaves was caused by a total of four (4) bacterial species and five (5) fungal species. The bacteria isolates were Bacillus subtilis, Serratia marcescens, Lactobacillus sp, and Proteus mirabilis. The fungal isolates were Aspergillus niger, Aspergillus flavus, Mucor mucedo, Fusarium oxysporum and Penicillium sp. The spinach had high moisture content ranging from 90.50% in the white variety (Basella alba) and 90.00% in the purple variety (Basella rubra). The presence of these microorganisms in the leafy spinach indicated microbial contamination, hence spoilage and loss of quality.

Deterioration; Spoilage; Contamination; Indian spinach
Indian spinach was described as a highly succulent vegetable similar to waterleaf [1]. It belongs to the family basellaceae. The name for spinach is derived from the perison word “ispanai” which means “greenhand” and which later became “spanachia” (Latin), to spinach in English Language [2], of the many spinach cultivars, Indian spinach is the most cultivated in Africa [3]. This is probably due to good environmental factors such as high temperature conditions, high rainfall and moist fertile soils with high organic matter observed in the African continent. The vegetable is cultivated for the use of its leaves and therefore used for soup or stew making. It contains large amounts of ascorbic acid and very rich in mineral substances [3]. In fact, according to Marjorie [5], a serving of 100 grams of Indian Spinach gives 3.2grams of protein, 4.3grams of carbohydrates and 0.3grams of fat together with various proportions of difference mineral elements. Basella alba and Basella rubra were reported as the two major species of Indian spinach in Africa with the former having green stems and green leaves while the latter has purple stems and purple leaves [3]. Spoilage of green leafy vegetable is due to the activity of microorganisms, the condition favourable for their proliferation being moisture and warmth [6].
Eboh and Okoh [6] has worked on microorganisms associated with some common vegetables in Nigeria excluding Basella species. Moreso, Adebayo [7] has equally worked on proximate composition and microbial spoilage of six selected leafy vegetables in South West, Nigeria in which Basella species was excluded out of his research work. Research programmes on leafy vegetables in Nigeria cover only the routinely cultivated ones such as Amaranthus sp (L.), Solanum macrocarpon (L), Celosia argentia (L.), Corchorus Oitorius (L), Telfairia occidentalis (F.), Hooks and Hibiscus sabdarifa (L.) [8]. So, there is dearth of information on the spinach vis-à-vis its spoilage and microbes associated with its deterioration. This study therefore, focuses on microorganims associated with the deterioration of fresh leafy Indian Spinach in storage.
Materials and Methods
Sources of sample
Two species of Basella namely Basella alba and Basella rubra were self – cultivated and hand harvested 90 days after planting. Before use, the fresh leaves were selected for uniformity of colour, smoothness, maturity based on size and kept unpreserved and unprocessed in polyethylene bags for 3 days before investigation. The leaves were stored in the bags according to their species type under ambient storage conditions.
Culture media
The culture media used for the isolation of bacteria was nutrient agar (oxoid) while potato dextrose agar (oxoid) used for the isolation of fungi was acidified with 1% lactic acid. The culture media used was made by Lab M Limited Topley House, 52 Washlane, Bury, Lancashire BLA 6 AS, United Kingdom.
Microbiological analyses
Microbial isolation and count carried out on the leafy samples by serial dilution plate method. The leafy samples randomly selected from each bag was immersed in sterile water and serially diluted to X10-3 dilution factor. The X10-3 dilution was plated in the respective media in triplicates using pour-plate technique for both enumeration and isolation. The isolates were subcultured to obtain pure cultures. The plates were incubated at 30°C for 24 hours and 72 hours for bacteria and fungi respectively. The bacterial isolate was identified using morphological and biochemical characteristics [9]. The moulds were identified using microscopy and cultural characteristics using the text of Frazier and Westhoff [10] and Alexopolus and Mims [11].
Moisture content determination
The moisture contents of the selected freshly harvested samples were carried out using the oven method. Each test sample was weighed accurately into standard dishes of known weights with tight fitting lids. The open dishes containing the samples were placed in oven at 105°C for 5hrs [12]. The dishes were then removed, covered with lids and allowed to cool in a dessicator before they were weighed to obtain the final weight. This was done in triplicates.
The moisture content of the test sample was then calculated as follows:
Table 1 showed the occurrence of bacteria and fungi on the deteriorated leafy spinach. The deterioration according to this work was evident by loss of green colour to mushiness of the leaves and very high microbial count. This is in agreement with the work of Kendal et al. [13] who reported loss of green pigments as a post harvest deterioration of leafy vegetables and microorganisms as agent of deterioration. Hence, the microbial isolates are implicated in the colour change of the leaves.
The various bacteria isolates associated with the deteriorated leafy spinach samples were Bacillus subtilis, Serratia marcescents, Lactobacillus sp and Proteus mirabilis while the fungal isolates included Aspergillus niger, Mucor mucedo, Aspergillus flavus and Fusarium oxysporum (Table 1). This agrees with reports of Anon [14] that growth and activity of microorganisms principally bacteria and fungi is one of the major causes of deterioration of leafy vegetables. The work of Eboh and Okoh [6] reported Fusarium sp, Aspergillus flavus, Aspergillus niger and Mucor sp as the organisms found associated with decayed leafy vegatables. So, the deterioration of the leafy spinach samples is not unconnected with these isolates.
All the bacterial and fungal isolates were found on both species of the spinach. Table 2 showed the total viable bacterial and fungal count of the spinach samples. The high total viable bacteria and fungal counts in the two species may not be unconnected with the high moisture contents of the spinach (Table 3) and moreso, that the vegetable is especially rich in vitamins and mineral substances. This validates the reports of Anon [14] that microbes grow in an environment of high moisture contents and nutrients. Thus, the high moisture contents of the Basella species coupled with its richness in minerals and vitamins must have encouraged the growth of this microbe on the spinach leading to its deterioration.
The emergence of the fungal isolates on the spinach samples no doubt suggests possible contaminations by spores in the air, since their spores are numerously available in the air [15]. The isolation of Staphylococcus aureus is indicative of human contamination during handling, since the microbe is known to be a normal flora of man (Onuorah et al, 1987). The presence of Bacillus subtilis, Serratia marcescens, Lactobacillus sp and Proteus mirabilis on the deteriorated spinach samples may be linked to the fact that these microbes are widely distributed in air, dusts and soils.
The high incidence of microorganisms in the spinach samples after the 3-day storage suggests spoilage and loss of eating quality of the spinach. This is in conformity with the reports of Kendall [16] that spinach could only be stored after harvest for one to two days in order to keep its eating quality, otherwise it would perish very quickly. However, the vegetable (Basella species) can be stored for a long time or has longer shelf life if preserved.
My foremost gratitude goes to the most High God for His mercy, wisdom, intervention and provision. In the same vein, sincere gratitude also goes to Dr. (Mrs.) A.T. Aborisade whose advice, kindness, gentle and thorough but intelligent correction and editing have immensely contributed towards the successful completion of this work. Lack of space and time will fail me to keep mentioning names. However, I deeply appreciate every contribution made by every individual in converting this research work into paper work, thus contributing my own quota to scientific knowledge.



 Download    pdf version of this article


Tables at a glance

Table 1  Table 2  Table 3
Select your language of interest to view the total content in your interested language
Share This Article
Relevant Topics
Disc Anthrax
Disc Aromatic Compounds
Disc Aromatic Crops
Disc Aromatic Flowers
Disc Aromatic Plants
Disc Aromatic Shrubs
Disc Aromatics
Disc Bacteraemia
Disc Bacterial Diseases of Plants
Disc Bacterial Ecology
Disc Bacterial Genomics
Disc Bacterial Infections
Disc Bacterial Toxin
Disc Bacteriology
Disc Basmati Rice
Disc Common Medicinal Plants
Disc Drought Tolerence
Disc Enzyme Activity
Disc Epidemeology of Plant Diseases
Disc Escherichia coli
Disc Essential Plant Pathology
Disc Fragrant Plants
Disc Gene Silencing
Disc Germination
Disc Golden Rice
Disc Green Chemistry
Disc Hawaiian Medicinal Plants
Disc Healing Herbs
Disc Healing Plants
Disc Herbal Monograph
Disc Herbs Side Effects
Disc Herbs Toxicity
Disc History of Medicinal Plants
Disc Host-Pathogen Interactions
Disc Intestinal Parasites
Disc Leaf Diseases
Disc Leprosy
Disc Listeriosis
Disc Long Grain Rice
Disc Lyme Disease
Disc Medicinal Herbs
Disc Medicinal Plants
Disc Medicinal Plants List
Disc Molecular Plant Pathology
Disc Native American Medicinal Plants
Disc Nematode Parasites
Disc Nucleic Acid
Disc Par Boiled Rice
Disc Parasitic Infection
Disc Parasitic Worms
Disc Parasitology
Disc Photosynthesis
Disc Phytochemical
Disc Plant Biochemistry
Disc Plant Biotechnology
Disc Plant Defensins
Disc Plant Diseases
Disc Plant Fungi and Diseases
Disc Plant Medicine
Disc Plant Pathology
Disc Plant Pathology Journal
Disc Plant Pheromones
Disc Plant Spirit Medicine
Disc Psychoactive Herb
Disc Raw Rice
Disc Rice
Disc Rice Blast
Disc Rice Bran
Disc Rice Diseases
Disc Rice Economics
Disc Rice Genome
Disc Rice Research Journals
Disc Rice Research Papers
Disc Rice Yield
Disc Rice and Aquaculture
Disc Rice and Nutrition
Disc Rice husk
Disc Rice production
Disc Rice research
Disc Rickettsioses
Disc Root Nematodes
Disc Salmonella
Disc Salmonellosis
Disc Sticky Rice
Disc Stress Resistant Rice
Disc Terminator Technology
Disc Transcriptomics
Disc Transgenic Plants
Disc Unpolished Rice
Disc Viral Diseases of Plants
Disc White Rice
Disc Zoonotic Bacterial Diseases
Related Journals
Disc Medicinal & Aromatic Plants Journal
Disc Rice Research Journal
Disc Bacteriology & Parasitology Journal
Disc Plant Biochemistry & Physiology Journal
Disc Plant Physiology & Pathology Journal
Related Conferences
Disc Plant Genomics Conference
July 14-15, 2016, Brisbane, Australia
Disc 5th Agriculture & Horticulture Conference
June 27-29, 2016, Cape Town, South Africa
Disc Plant Physiology Conference
June 9-11, 2016, Dallas, USA
Disc 2nd Plant Science Conference
October 31-November 02, 2016, Baltimore, USA
Article Tools
Disc Export citation
Disc Share/Blog this article

Post your comment

Your question:
Anti Spam Code:
  Reload  Can't read the image? click here to refresh

OMICS International Conferences 2015-16

Meet Inspiring Speakers and Experts at our 1000+ Global Annual Meetings
Conferences By Country
  USA   Spain   Poland
  Australia   Canada   Austria
  UAE   Switzerland   Turkey
  Italy   France   Finland
  Germany   India   Ukraine
  UK   Malaysia   Denmark
  Japan   Singapore   Mexico
  Brazil   South Africa   Norway
  South Korea   New Zealand   China
  Netherlands   Philippines
Medical & Clinical Conferences
Microbiology Oncology & Cancer
Diabetes & Endocrinology Cardiology
Nursing Dentistry
Healthcare Management Physical Therapy Rehabilitation
Neuroscience Psychiatry
Immunology Infectious Diseases
Gastroenterology Medical Ethics & Health Policies
Genetics & Molecular Biology Palliativecare
Pathology Reproductive Medicine & Women Healthcare
Alternative Healthcare Surgery
Pediatrics Radiology
Conferences by Subject
Pharmaceutical Sciences
Pharma Marketing & Industry
Environmental Science
Physics & Materials Science
EEE & Engineering
Chemical Engineering
Business Management
Geology & Earth science
©2008-2015 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version