ISSN 2469-9853

Journal of Next Generation Sequencing & Applications
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About the Journal

Journal of Next Generation Sequencing & Applications is a peer reviewed medical journal that includes a wide range of fields in its discipline to create a platform for the authors to make their contribution towards the journal and the editorial office promises peer review for the submitted manuscripts to ensure quality. Sequencing is the process of determining the precise order of nucleotides within the genome. It includes various methods or technologies that are used to determine the order of the four nucleotides in the DNA strand. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.

Journal of Next Generation Sequencing & Applications is a broad-based journal found on two key tenets: To publish the most exciting Reviews on Next generation Sequencing & Applications: Second to provide a rapid turn-around time possible for reviewing and publishing of articles freely for research, teaching and reference purposes.It is basically aimed at the Clinical Practitioners, medical/ health practitioners, students, professionals and researchers and professional bodies and institutions.

This scholarly publishing is using Editorial Manager System for quality in the review process. Editorial Manager is an online manuscript submission, review and that tracks the review status. Review process is performed by the editorial board members of Journal of Molecular Biomarkers & Diagnosis or outside experts; at least two independent reviewer’s approval followed by the editor is required for the acceptance of any citable manuscript.

Paired End Sequencing

Paired end sequencing is defined as a process to sequence both ends of the fragment and to generate high sequence data. Paired end sequencing detects the genomic rearrangement, repetitive sequence elements, gene fusion and novel transcripts. Since paired-end reads are more likely to align to a reference, the quality of the entire data set improves. All Illumina NGS (next-generation sequencing) systems are capable of paired-end sequencing. Paired-end DNA sequencing reads provide superior alignment across DNA regions containing repetitive sequences, and produce longer contigs for de novo sequencing by filling gaps in the consensus sequence.

Related Journals of Paired End Sequencing

Journal of Next Generation Sequencing & Applications, Advancements in Genetic Engineering, Journal of Computer Science & Systems Biology, Journal of Proteomics & Bioinformatics, Transcriptomics: Open Access, Journal of Bioinformatics and Sequence Analysis, Sequencing Oxford Journals, International Journal of Paired Sequencing, European Journal of Paired Sequencing, Bioinformatics Journal of Sequencing

16S ribosomal RNA

16s ribosomal RNA  is a small subunit of 30S ribosome in prokaryotes. The gene coding for 16S ribosomal RNA is 16 S rRNA. It is used for reconstruction of phylogenies. In a single pacterium, multiple sequences of 16S rRNA can exist. 16S ribosomal RNA (rRNA) sequencing is a common amplicon sequencing method used to identify and compare bacteria present within a given sample. 16S rRNA gene sequencing is a well-established method for studying phylogeny and taxonomy of samples from complex microbiomes or environments that are difficult or impossible to study.

Related Journals for 16S ribosomal RNA

Journal of Next Generation Sequencing & Applications, Advancements in Genetic Engineering, Journal of Computer Science & Systems Biology, Journal of Proteomics & Bioinformatics, Transcriptomics: Open Access, Society for general Microbiology Journals, Journal of Ribosomal RNA & Sequencing, Archives of  Ribosomal RNA, Ribosomal RNA & Sequencing Review.

Nucleotide Sequencing

Nucleotide sequencing is defined as a process of determining the order of nucleotides. Nucleotides are defined as an organic molecules that acts as monomers or the subunits of nucleic acids. Nucleotides are known as the building blocks for nucleic acids. Nucleotides are composed of nitrogenous base, a five carbon sugar that is ribose or deoxyribose and at least one phosphate group.

Related Journals for Nucleotide Sequencing

Journal of Next Generation Sequencing & Applications, Advancements in Genetic Engineering, Journal of Computer Science & Systems Biology, Journal of Proteomics & Bioinformatics, Transcriptomics: Open Access, Journal of Sequencing and Mapping, Nucleotide Sequence Databases Oxford Journal, International Journal of DNA Sequencing.

Transcriptome sequencing

Transcriptome is defined as the set of RNA molecules in a cell. RNA molecules includes mRNA, tRNA and rRNA. It is a revolutionary tool that offers many advantages over the traditional microarray-based gene expression approaches Transcriptome sequencing helps in understanding of molecular mechanisms and then it tells about signating pathways which controls embryonic development. Transcriptome analysis may involve characterization of all transcriptional activity, or a select subset of RNA transcripts within a given sample.

Related Journals for Transcriptome Sequencing

Journal of Next Generation Sequencing & Applications, Advancements in Genetic Engineering, Journal of Computer Science & Systems Biology, Journal of Proteomics & Bioinformatics, Transcriptomics: Open Access, Integrated Genome and Transcriptome Sequencing Journal, Journal of Marine Microbial Eukaryote Transcriptome Sequencing, An RNA sequencing Transcriptome Journal, Oxford Journals for Transcriptome.

Sanger Sequencing

Sanger sequencing is defined as a method of DNA sequencing which is based on incorporation of chain- terminating dideoxynucleotides by DNA polymerase during in vitro  DNA replication. Sanger sequencing by capillary electrophoresis is the gold-standard DNA sequencing technique that is used in a number of experimental workflows in life sciences laboratories. In Sanger sequencing, DNA polymerases copy single-stranded DNA templates by adding nucleotides to a growing chain. Chain elongation occurs at the 3' end of a primer, an oligonucleotide that anneals to the template. The deoxynucleotides added to the extension product are selected by base-pair matching to the template.

Related Journals for Sanger Sequencing

Journal of Next Generation Sequencing & Applications, Advancements in Genetic Engineering, Journal of Computer Science & Systems Biology, Journal of Proteomics & Bioinformatics, Transcriptomics: Open Access, Frederick Sanger sequencing Journal, Sanger Sequencing Biosystems, Methods of Sanger Sequencing, Nucleic acids Research Oxford Journals.

Pyro Sequencing

Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle. It differs from Sanger sequencing, in that it relies on the detection of pyrophosphate release on nucleotide incorporation, rather than chain termination with dideoxynucleotides. It detects pyrophosphate release on nucleotide rather than chain termination with dideoxynucleotides.

Related journals for Pyro Sequencing

Journal of Next Generation Sequencing & Applications, Advancements in Genetic Engineering, Journal of Computer Science & Systems Biology, Journal of Proteomics & Bioinformatics, Transcriptomics: Open Access, The ISME Journal of Pyro sequencing, Microbial Analysis Journals, Pyro Hard Cover Journals, Journal of Analytical & Applied Pyrolysis, Journal of Pyrotechnics Archive.

Maxam gilbert sequencing

Maxam Gilbert sequencing is defined as a method of DNA sequencing which is based on nucleobase- specific partial chemical modification of DNA and subsequent of DNA cleavage. Nucleobase is defined as compounds containing nitrogen groups. In a Maxam and Gilbert sequencing, the identity of guanine or cytosine in the sequence can be assigned most easily because two of the four reaction sets cleave at bases alone.

Related Journal for Maxam gilbert sequencing

Journal of Next Generation Sequencing & Applications, Advancements in Genetic Engineering, Journal of Computer Science & Systems Biology, Journal of Proteomics & Bioinformatics, Transcriptomics: Open Access, Maxam gilbert sequencing research, Adaption of Maxam & gilbert sequencing methods, Haskins society Journals.

Next generation sequencing machines

Next generation sequencing machines or instruments are as mentioned as DNA microarrays, real-time PCR and DNA chips and reagents. Data analysis software. They are used for instrumentation of DNA sequencing. Next-generation sequencing, also known as high-throughput sequencing, is the catch-all term used to describe a number of different modern sequencing technologies including: Illumina (Solexa) sequencing, Roche 454 sequencing, Ion torrent: Proton / PGM sequencing, solid sequencing.

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Journal of Next Generation Sequencing & Applications, Advancements in Genetic Engineering, Journal of Computer Science & Systems Biology, Journal of Proteomics & Bioinformatics, Transcriptomics: Open Access, Journal of American Medical Association, British Medical Journal, Astrophysical Journal, Genome Mapping & Genomics in Animals, Law & the Human Genome, Annals of Internal Medicine.

Sequencing primers

Sequencing primers are defined as strand of short nucleic acid sequences that leads as a starting point for DNA synthesis. It is required for DNA polymerisation and DNA replication. The polymerase starts at 31 - end of primer and copies the opposite strands. The primer sequence must be unique to the region you wish to sequence and it must be in the correct orientation.It must not contain undesirable self-hybridization. Generally, primers that can form four or more consecutive bonds with themselves are called self-hybridized primers.

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Journal of Next Generation Sequencing & Applications, Advancements in Genetic Engineering, Journal of Computer Science & Systems Biology, Journal of Proteomics & Bioinformatics, Transcriptomics: Open Access, Primer design for large scale sequencing, PCR direct sequencing, Sequence Extractor Bioinformatics, Addgene sequencing primers.

Denovo sequencing

Denovo sequencing is defined as a peptide sequencing. Peptide sequencing is defined as the sequencing of peptide bonds present in the organism.it is carried out by using shortgun libraries and long paired- end libraries. The initial generation of the primary genetic sequence of a particular organism is called denovo sequencing. A detailed genetic analysis of any organism is possible only after denovo sequencing has been performed. De novo sequencing is typically accomplished by assembling individual sequence reads into longer contiguous sequences or correctly ordered contigs in the absence of a reference sequence.

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Journal of Next Generation Sequencing & Applications, Advancements in Genetic Engineering, Journal of Computer Science & Systems Biology, Journal of Proteomics & Bioinformatics, Transcriptomics: Open Access, Journal cover features Denovo sequencing, Single-read Denovo Sequencing, Denovo peptide Sequencing Journal, Journal of Neuroscience, Journal of Immunology.

Metagenomics

Metagenomics is defined as branch of genetics which deals with the study of genetic material from environmental samples. It is the genomic analysis of microorganisms by direct extraction and cloning of DNA. Metagenomics deals with the study of  microorganisms which cannot be cultured. Metagenomics has emerged as a powerful tool that can be used to analyze microbial communities regardless of the ability of member organisms to be cultured in the laboratory.

Related journals for Metagenomics

Journal of Next Generation Sequencing & Applications, Advancements in Genetic Engineering, Journal of Computer Science & Systems Biology, Journal of Proteomics & Bioinformatics, Transcriptomics: Open Access, ISME Journal, Journal of Biology Chemistry, Bioinformatics, Tree Genetics & Genomes, Genome Biology and Evolution, Cytogenic and Genime research.

Next generation sequencing

Next generation sequencing is defined as a process where DNA strands are sequenced parallel and substantially minimize need for the fragment cloning which are used in sanger sequencing of genome. There are different technologies used in next generation sequencing. The high demand for low-cost sequencing has driven the development of high-throughput sequencing, which is also termed as Next generation sequencing (NGS). Thousands or millions of sequences concurrently produced in next-generation sequencing process. Next generation sequencing has become a commodity.

 Related Journals for Next generation sequencing

Journal of Next Generation Sequencing & Applications, Advancements in Genetic Engineering, Journal of Computer Science & Systems Biology, Journal of Proteomics & Bioinformatics, Transcriptomics: Open Access, American journal of Respiratory and Critical Care Medicine, Journal of Clinical Endocrinology and Metabolism, Journal of National Cancer Institute, EMBO Journal.

454 sequencing

454 sequencing is defined as a process of sequencing where nucleotides are flowed sequentially in a fixed order across the pico -titer plate device during a sequencing run. DNA molecules are sequenced in parallel. The pico-titer plate device is used for 454. 454 Sequencing is based on sequencing–by–synthesis where nucleotides are flowed sequentially in a fixed order across the Pico Titer Plate device during a sequencing run. During the nucleotide flow, hundreds of thousands of beads each carrying millions of copies of a unique single–stranded DNA molecule are sequenced in parallel.

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Shotgun sequencing

Shotgun sequencing is the method that was used by the private genome project. Shotgun sequencing requires multiple copies of the genome, which are effectively blown up into millions of small fragments. It is carried out as large DNA strand and is broken into small fragments , and they are reassembled according to their overlaps. Then complete clone of DNA is formed.

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Genome Sequencing Technique

Genome sequencing is defined as a process of sequencing of genomes. Genome is defined as a total amount of genetic information present in a chromosome of an organism. Chromosomes consists of different types of chromosomal sets they are as follows: a. Haploid set: single set of chromosomes present in an organism. It is present in eukaryotes.b. Diploid set: organisms containing two sets of chromosomes. It is present in prokaryotes. For whole-genome sequencing, the combination of short inserts and longer reads allow characterization of any genome.

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Journal of Next Generation Sequencing & Applications, Advancements in Genetic Engineering, Journal of Computer Science & Systems Biology, Journal of Proteomics & Bioinformatics, Transcriptomics: Open Access, Genomics Journal Elsevier, Journal of Genomics, Whole Genome Sequencing Journal, International Journal of Genomic Sciences, Genome Integrity, Genome Dynamics.

Journal of Next Generation Sequencing & Applications is associated with our international conference "3rd International Conference on Genomics & Pharmacogenomics" during September 21-23, 2015 San Antonio, USA with a thematic blend of "Implications and Impacts of Genomic Advances on Global Health" We are particularly interested in research area Genetics, Bioinformatics, Proteomics, Pharmacogenomics and Bio-Engineering etc.

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