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In Association with
Affect of heavy metal tolerance induced oxidative stress on energy metabolism in Drosophila melanogaster
6th World Congress on Biotechnology
Geetanjali Sageena, Rakesh Roshan, Shreya Chaudhary, Nalini Mishra and Mallikarjun Shakarad
University of Delhi, India
ScientificTracks Abstracts: J Biotechnol Biomater
Abstract
Holometabolous insect, Drosophila melanogaster serves as an exemplary model system to study impact of environmental stress on both energy acquisition and allocation as it comprises of two distinct phases of life cycle, namely, growing preadult phase and post mitotic adult phase. Resource acquisition in D. melanogaster occurs during the three larval stages of the pre-adult phase while resource allocation towards adult survival and reproduction occurs perhaps during the differentiating pupal stage as well as in the early adult phase. Through this study we tried to assess the affect of heavy metals in the larval diet on energy budget of the adult flies, in response to exposure of growing larvae to diet laced with specific heavy metals as seen in energy depleted populations of D. melanogaster as a result of selection for faster pre-adult development and compare with changing energy dynamics of normal fly population. Our results show that energy compromised yet long lived flies have reduced stress tolerance compared to normal flies, reaffirming the finding that stress tolerance and adult lifespan are not tightly correlated (F1, 2=50.843, p=0.01). Further, there was significant effect of treatment (F2, 4=16.107, p=0.01) and gender (F1, 2=32.818, p=0.02), in that the females had higher levels of energy than males. The fly’s enclosed from heavy metals laced diet had lesser energy accumulated than those reared on SM. The results clearly demonstrate the dynamic nature of relationship between different life history traits such as longevity and stress resistance. Further, this study also established the fact that the relationship between life-history traits are strongly mediated through internal energy levels of the flies.Biography
Geetanjali Sageena has submitted her PhD thesis in April 2015 from the Department of Zoology, University of Delhi, India. She is currently working as an Assistant Professor at Keshav Mahavidyalaya, an undergraduate college of University of Delhi.
Email: cutesmile.pie@gmail.com
