Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
GET THE APP
Application Of Supercritical Fluid Chromatography/mass Spectrometry To Metabolic Profiling | 10168
ISSN: 2155-9872
Journal of Analytical & Bioanalytical Techniques
Open Access
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Owing to its favorable properties such as low viscosity and high diffusivity, a supercritical fluid can be used as the mobile
phase in chromatography. Supercritical fluid chromatography (SFC) can provide high-speed and high-resolution separation.
Because supercritical carbon dioxide (SCCO
2
), which is generally used as the mobile phase in SFC, is automatically emitted at
room temperature, SFC is most commonly used as a preparative method. However, SFC can also be used to perform high-
precision biomolecular analysis, especially for hydrophobic metabolites, because of the low polarity of SCCO
2
. The use of a mass
spectrometer (MS) with SFC can widen the scope of application of SFC to bioanalysis. Therefore, we have tried to apply SFC/MS
to a metabolic profiling.
We developed a useful workflow using SFC, a quadrupole orbitrap mass spectrometer, which can perform high-resolution
full scanning and product ion scanning in both the positive-ion mode and the negative-ion mode in practical cycle time,
and an automated lipid identification system. Additionally, we developed a polar lipid profiling method by SFC/MS with
trimethylsilylation and methylation. The targeted lipid profiling by SFC/MS was also constructed. SFC/MS was useful for the
separation of lipids having numerous analogs with similar structures, such as carotenoids and triacylglycerols. Additionally, SFC/
MS showed the maximum efficiency for the analysis of a biological sample that includes many matrices. The high-resolution,
high-throughput analytical system based on SFC/MS is suitable for studies on lipid metabolomics because it is useful not only for
the screening of diverse lipids (as a fingerprint method) but also for the detailed profiling of individual components. Furthermore,
SFC/MS is applicable for the analysis of various metabolites besides lipids and is expected to emerge as a powerful analytical tool
in metabolomics.
Biography
Takeshi Bamba is an Associate Professor at Department of Biotechnology, Graduate School of Engineering, Osaka University, Japan. He has
completed his Ph.D. from Osaka University. His laboratory focuses on the development of metabolomics technology including data mining and its
application research in various fields. He has published more than 100 articles in scientific journals/books.
Relevant Topics
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals