Journal of Biotechnology & Biomaterials
Open Access

Abstract

The biodiesel production is the transesterification reaction which could be catalyzed either chemically or enzymatically. Enzymatic transesterification has certain advantages over the chemical catalysis of transesterification, as it is less energy intensive, allows easy recovery of glycerol and the transesterification of glycerides with high free fatty acid contents. Lipases (triacyl glycerol acylhydrolase EC 3.1.1.3) are hydrolytic enzymes that catalyze the hydrolysis and synthesis of variety of acylgcerols at interface of lipid and water. In the present study, transesterification reaction of karanjia oil was carried out by using Pseudomonas aeruginosa lipase. In this study, the effect of various parameters were optimized. Enhanced conversion 85.9 % of fatty acid to fatty acid methyl ester within 48 h at 45 ?C was achieved by using lipase at a concentration of 5 % (w/v of oil), molar ratio of 1:4 (oil: methanol), methanol acyl donor, n-hexane solvent and temperature 45 ?C. The biodiesel obtained was qualitatively determined by thin layer chromatography and gas chromatography. This approach might pave the way for industrial production of biodiesel equivalents from renewable resources by employing micro ? organism, enabling a broader use of biodiesel ? like fuel in the future.

Biography

Vinod Kumar has been a student at University of Delhi South Campus. He is pursing Ph.D. in Microbiology, under the guidance of Prof. R. K. Saxena at Department of Microbiology. His main research subject is to develop an Eco friendly system for Synthesis of bioactive molecules by enzymatic method which important in industrial sector. He has published 5 papers in reputed journals and papers presented in National/International conferences.