Clinical Microbiology: Open Access

Characterization of dye decolorization by cell-free culture broth of Trametes vesicolor CBR43

3^rd Global Microbiologists Annual Meeting

August 15-17, 2016 Portland, Oregon, USA

Hee Wook Ryu, Hyun Ryu and Kyung-Suk

Soongsil University, South Korea
Korea University, South Korea
Ewha Womans University, South Korea

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Trametes versicolor CBR43 was isolated from Chunma Mountain, South Korea. The fungus had high activities of laccase and Mn dependent peroxidase whereas its lignin peroxidase activity was relatively low. T. versicolor CBR43 could decolorize 200 mg/L of acid dyes (red 114, blue 62, orange 7 and black 172), reactive dyes (red 120, blue 4, orange 16 and black 5) and disperse dyes (red 1, orange 3 and black). Except acid orange 7, T. versicolor CBR43 decolorized more than 90% of acid and reactive dyes within 6 days in the PDB medium. This fungus decolorized 67% of acid orange 7 within 9 days. The decolorization efficiencies for disperse dyes were 51, ~80% at 9th day. The maximum rates of dye decolorization in the cell-free culture broth of T. versicolor CBR43 were 1,410, 44.7, 41.2 and 0.19 μmol L-1 min-1 for acid blue 62, acid black 175, reactive blue 4 and acid red 114, respectively. The inhibition effect of NaCl, Zn(II) and Cd(II) on the dye decolorization has been quantitatively compared using the half maximal inhibitory concentration (IC50) indicating the concentration of an inhibitor that is required for 50% inhibition. Based on the values of IC50, the dye decolorization in the cell-free culture broth of CBR43 was most severely inhibited by Cd(II), and the inhibition effect of NaCl was relatively low. The information on dye decolorization rates and IC50 can be utilized to design for the development of a dye wastewater treatment process employing T. versicolor CBR43 and its operating factors.

Biography :

Hee Wook Ryu has completed his PhD from KAIST and Postdoctoral studies from University of Minnesota. He is currently a Professor at Soongsil University, South Korea. He has published more than 70 papers in reputed journals.

Email: hwryu@ssu.ac.kr