Clinical Microbiology: Open Access

Correlation between phage typing and toxins content as an outbreaks tool in Staphylococcus aureus

3^rd International Conference on Clinical Microbiology & Microbial Genomics

September 24-26, 2014 Valencia Convention Centre, Spain

Nagwa Mohamed Amin Aref, Manal M Al Khulaifi, Ali A Al Salamah and Mohammed S Al-Shammary

Accepted Abstracts: Clin Microbial

Abstract:

Objectives: To evaluate spread of MRSA in Saudi cohort by examining phage typing and genetic backgrounds toxins. As a phenotypic marker to find out the existence S. aureus strains that cause a significant proportion of sporadic human infections EMSSA. Methods: A total number of 119 S. aureus isolated from different specimens were obtained over a period of one year from 2003 to 2004 from microbiology laboratory in Riyadh Military Hospital, and were studied for phage typing and the incidence of toxin genes by PCR. Results: MRSA indicated high special prevalence of phage group II with a high increase for phage type ?3A compared to MSSA. Phage group II on MSSA considered an epidemiologic marker with frequent strong reaction compared to group III and phage group I. Phage type ?75 play an important role in a combination with ?80 or/ ?81 to acquire SCCmec directly or via phage type ?80/81 by having PVL toxin to be CMRSA lineages. 68% of S. aureus isolates had toxins. The most prevalent toxins were SEO, in 50.8% in MSSA & 25% in MRSA. SEI was detected in 40.3% in MSSA & 29.1% in MRSA. Also, SEA was 28% in MSSA & 33.3% in MRSA. Conclusion: Phenotypic and genotypic variations between MSSA isolates seemed to be horizontally, while in MRSA were vertically. It was obvious that 5 toxins together were located in more than one isolates in MSSA.

Biography :

Nagwa Mohamed Amin Aref has completed her PhD in Ain Shams University, Cairo, Egypt as a channel system study with University of Hohenheim, Institute fur phytomidizine, Stuttgart, Germany. In 1984, in Virology and Virus diagnoses and postdoctoral studies from Michigan State University, Department of Botany and Plant Pathology and Center of Electron Optics in East Lansing, USA, participating in the detection of Plant viral disease of the research program 1984-1989. Coinvestigated at AGERI (Agriculture Genetic Engineering Research Institute), Ministry of Agriculture, Egypt, 1990-1996 in UNDP project entitled: ?Engineering tomato transgenic plants resistant? in contribution with Scripps Research Institute, San Diego, USA. She was an Exchange Visitor Professor for a research scholarship in Electron Imaging Center, California NanoSystem Institute (CNSI, University of California (UCLA) in Los Angeles, USA. She has published more than 40 publications in reputed journals supervising postgraduate students and serving as an editorial board member of reputed journals. She is working now as a Professor in Botany and Microbiology Department in College of Science, King Saud University.