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Development and validation of new clean-up approach for 21 organo-chlorinated pesticides (ocps)
3rd International Conference and Exhibition on Analytical & Bioanalytical Techniques
Amel Al-Rashdan and Murad I.H. Helaleh
ScientificTracks Abstracts: J Anal Bioanal Techniques
Abstract
The separation efficiency of micellar solutions in thin layer chromatography of hydrophilic vitamins (B 1 , B 9 , B 12 and vitamin C) has been examined using silica gel as stationary phase. The best results were obtained with mixed micelles (aqueous solution of anionic SDS (0.5%) plus non-ionic Triton X-100 (0.5%) surfactants) and butanol (9:1 v/v) as mobile phase. The proposed method is applicable to the identification and separation of vitamins (B 1 , B 9 and vitamin C) present in drug samples (Becasule and Celin). The separation 1 μg of vitamin C from milligram quantities (up to 0.50 mg) of vitamins B 1 has been successfully realized. Effect of metal ions as impurities, limit of detection and semi quantitative analysis on the separation of vitamins have been examined.Biography
Sudhanshu Sharma has completed his Ph.D. in Applied Chemistry on ?Surfactant Mediated Chromatographic Analysis of Pharmaceuticals? from Aligarh Muslim University, Aligarh, India at the age of 27. He has published more than 12 Research papers in reputed journals and serving as an editorial board member of various repute Journals during last 03 Years. He is involved in various research projects based on application of nano- materials, surfactants and right now working on application of silver nano particles in cancer prevention. Currently he is working as an Assistant Professor at Department of Applied Chemistry, O. P. Jindal University Raigarh, India.
