Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Wataru Aoki and Mitsuyoshi Ueda
Accepted Abstracts: J Proteomics Bioinform
Candida albicansis an opportunistic pathogen that causes fatal disease if the host immunity is compromised. The mortality rate of systemic candidiasis is very high; hence, there is a ceaseless demand for novel pharmaceuticals against C. albicans. In this study, quantitative time-course proteomics of C. albicans during adaptation to fetal bovine serum (FBS) is described. Survival in blood is essential for virulence of C. albicans, and a minute analysis of such a process is required. We cultivated C. albicansin FBS and buffered yeast extract-peptone-dextrose media for 0-180 min, and determined quantitative time-course variations of 1024 proteins in the cultured cells by using a nano-LC-MS/MS system with a long monolithic silica capillary column. A monolithic silica capillary column, prepared from a mixture of tetramethoxysilane and methyltrimethoxysilane in a long-fused silica capillary, shows higher performance compared to conventional particle-packed columns in separation of proteome samples. Clustering analysis identified FBS-induced proteins associated with virulent processes. Furthermore, we identified possible virulence factors relating to a pleiotropic stress-tolerance phenotype, indicating a role for quick adaptation to a stressful environment. Identification of candidate virulence proteins will lead to further understanding of C. albicans and to the development of novel antifungal agents.
After Wataru Aoki has obtained the doctorate degree from Kyoto University and the research fellowship of the Japan Society for the Promotion of Science (JSPS), a Japan?s leading funding agency, he has moved to Osaka University under the supports of the JSPS. He has published more than 15 papers in reputed journals and attended several international conferences.