Journal of Vaccines & Vaccination

Epitope based vaccine design against Japanese encephalitis virus.

International Conference & Exhibition on Vaccines & Vaccination

22-24 Nov 2011 Philadelphia Airport Marriott, USA

Bhawna Rathi

Scientific Tracks Abstracts: J Vaccines Vaccin

Abstract:

Japanese Encephalitis is a serious public health problem with signifi cant mortility in children and old people in Asian countries. JE virus, a fl avivirus (single-stranded RNA), represents the most signifi cant etiology of arboviral encephalitis worldwide. Th e current stategy discusses the scientifi c approach of T- lymphocyte epitope based vaccine. Th e concept of peptide-based vaccine holds several advantages over traditional vaccines, including safety considerations, the relatively long shelf-life, the ability to target the immune response towards specifi c epitopes that are neither suppressive nor hazardous for the host and the possibility of preparing multi-pathogen vaccine. Th e effi cacy of a peptide vaccine is highly dependent on the exact identifi cation of the immunogenic epitopes that confer protection as well as the effi cient presentation of these epitopes to the immune system. Th e study initially involves the identifi cation of components of, immunogenic peptides (epitopes) of JEV, which may be expected to mediate the immune response to this virus. In silico approaches are used for the design of EVs. In particular, computational methods for MHC binding prediction have already become standard tools in immunology. In silico approaches to MHC binding prediction yield high accuracies. Later, population coverage analysis will be done for the predicted epitopes to determine the proportion of various populations that may be expected to show T-cell response to each peptide. Th e conservancies of these predicted T-cell epitopes across various JEV genotypes will also assessed. At last, the revelant epitopes studied in the second part of the approach would be synthesized as chimeras. Th e chimeric peptide(s) will be expressed and purifi ed aft er cloning it into a suitable vector (pUC series / pET series). Th e chimeric peptides thus purifi ed would be studied for immugenic responses by ELISA and Western blot techniques

Biography :

Bhawna Rathi has completed her PhD work from SGPGI, Lucknow. She has been into research for 7 years. She has 6 international publications and attended many national and international conferences. Presently working as Lecturer at Amity University, India