Pharmaceutica Analytica Acta

Formulation and characterization of polymeric nanoparticles of Anti-tubercular drugs-Rifampicin, Isoniazid, Pyrazinamide and Ethambutol by modified solvent emulsification evaporation method

5^th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems

March 16-18, 2015 Crowne Plaza, Dubai, UAE

Maya N, Brahmeshwar Mishra and Ruchi Chawla

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

Tuberculosis (TB) is an infectious bacterial disease caused by Mycobacterium tuberculosis, which most commonly affects the lungs. The current therapy utilizes higher dose of drugs that cause dose related side effects. The aim of the present study was to formulate, optimize and characterize polymeric nanoparticles of anti-tubercular drugs- rifampicin (RIF), isoniazid (INH), pyrazinamide (PYZ) and ethambutol (ETH) to deliver the anti-tubercular drugs at a sustained rate. The optimization was done using central composite factorial design. The prepared nanoparticles were characterized for physico-chemical properties such as particle size, poly dispersity index (PDI) and zeta potential (ZP), % entrapment efficiency (%EE), total drug content and in vitro drug release. The optimized nanoparticles exhibited particle size of 244.1 nm, PDI of 0.299 and smooth and spherical surface morphology, with % EE of RIF, INH, PYZ and ETH 74.93%, 68.1%, 70.08% and 64.22% respectively. The ZP of the optimized batch of polymeric nanoparticles was found to be - 38.42+1.91 mV suggesting good stability. The release of drugs from nanoparticles was extended up to 72 hours, while, free drug solutions released 97% of the drugs in less than 3 hours. No significant difference (p<0.05) with respect to size, drug entrapment, PDI and ZP was observed between the stability batch and fresh batch during accelerated stability studies. The qualitative in vivo studies in alveolar macrophages suggested endocytosis of nanoparticles without any adsorption phenomenon. However, further studies should be carried out for establishing the pharmacokinetic potential of the polymeric nanoparticles.

Biography :

Maya N is a Post graduate student at Indian Institute of Technology (Banaras Hindu University), India. Her current area of research is Nano technology based drug delivery systems and Semi-solid dosage forms. Before joining as Postgraduate fellow, she completed Bachelor of Pharmacy from College of Pharmaceutical Sciences, Government Medical College, Calicut. She is the winner of All India Pharmacy Quiz Competition 2011 conducted by Government Madras Medical College, Chennai. She has qualified GPAT-2013 with All India Rank 38. She is receiving financial assistance, from MHRD, Government of India for her Postgraduation research work.