Journal of Clinical Toxicology
Open Access

Genotoxicity: Basic aspects and most commonly worldwide employed and validated in vivo assays

4^th Global Summit on Toxicology

August 24-26, 2015 Philadelphia, USA

Rohan Kulkarni

Scientific Tracks Abstracts: J Clin Toxicol

Abstract :

The in vivo genetic toxicology testing is performed usually in rodents such as rats or mice to determine the potential of
DNA damage that can affect chromosomal structure or mitotic apparatus such as spindle fibers that in turn could changes
chromosome number. In vivo assays can also detect genotoxic agents missed in in vitro tests. These assays are mainly divided
into two broad categories; either to detect chromosome damage or DNA damage. The positive result of induced chromosomal
damage is an increase in frequency of micronucleated PCEs or increase in chromosome aberrations. Micronucleus is a small
nucleus that forms whenever a chromosome or a fragment of a chromosome is not incorporated into one of the daughter
nuclei during cell division. This is typically caused by loss of chromosomal fragments due to clastogenicity or loss of entire
chromosome due to spindle fiber malfunction as a result of aneugenicity. The mammalian in vivo chromosome aberration test is
used for the detection of structural chromosome aberrations in bone marrow. The in vivo alkaline single cell gel electrophoresis
assay also called alkaline Comet Assay is a method measuring DNA damage. This Assay can be used to detect DNA damage
caused by double strand breaks, single strand breaks, alkali labile sites, oxidative base damage and DNA cross-linking with
DNA or protein. The basic principles underlying these assays and their basic design will be presented along with the regulatory
guidelines governing these assays.

Biography :

Rohan Kulkarni is currently serving as the Director of Genetic Toxicology-Study Management at BioReliance. He received BSc degree in Zoology from Mumbai
University, in 1998 and MSc degree in Zoology and Marine Science from Mumbai University in 2000. Before relocating to the US in 2003, he worked as a Product
Development Specialist at TechnoSource, a start-up Biotech company in India. He received his PhD from Wayne State University, MI in Biological Sciences
in 2008 under Dr. James D. Tucker. He received the Prestigious Alexander Hollander Travel award in 2003 at the 38th EMS Meeting in Atlanta. He completed
his Postdoctoral fellowship from National Center for Toxicological Research, US FDA in 2011 in the Division of Genetic and Molecular Toxicology. He joined
BioReliance in October 2011 as a Senior Scientist for in vivo and in vitro cytogenetic assays at BioReliance, with Study Director Responsibilities for GLP and non-
GLP cytogenetic studies. In 2015, he was appointed as the Director of Genetic Toxicology-Study Management. He has presented much of this work in several book
chapters, poster presentations and many journal articles. He is a full member of Society of Toxicology, Genetic Toxicology Association and an Associate Member
in the American College of Toxicology. He is also a member of the Genetic Toxicology Technical Committee organized by the ILSI-HESI group and a member of
the Core committee for in vivo follow-up testing.