Identification and the slignments of cancer-specific protein in lung tissues by capillary electrophoresis with laser-induced fluorescence

2^nd International Conference and Exhibition on Materials Science & Engineering

October 07-09, 2013 Hampton Inn Tropicana, Las Vegas, NV, USA

Rong Wang

Accepted Abstracts: J Material Sci

Abstract :

Capillary electrophoresis with laser-induced fluorescence (CE-LIF) is used for the identification and alignments of cancer- specific protein in lung tissues. CE-LIF is an effective method for proteomics study cause its high resolution, little sample consuming and automatic. Currently a number of methods are employed to identify protein, with the most common being LC-MS/MS and two-dimensional (2D) gel electrophoresis. However, the most obvious disadvantages include hard to alignment directly and inconvenient to quantitative analysis. CE-LIF technology with cancer protein detection need optimize the conditions of capillary coating, running buffer and derivatization reagent. We developed the optimum separation methods for lung carcinoma and adjacent normal tissue protein. Additionally, we firstly used the similarity evolution system for fingerprint caparison. Optimum separation conditions were developed the protein separation for lung carcinoma and adjacent normal tissue protein, which included 0.25% Polyethylene oxide (PEO) as sieving matrix, pH 10 buffer for avoiding protein adoption, fluorescein isothiocyanate (FITC) as pro-column derivatization. Electrophoretic separation was accomplished using 15.0 kV constant voltage, in 15 0 C. reverse polarity, and 1 ×TBE buffer for 20min. Based on the analysis of migration time and peak area, we evaluated the similarity of tumor and adjacent normal protein by similarity evaluation system for chromatogrphic fingerprint of TCM.It was showed that Tumor proteins distinguish normal protein with 17.1% and the similarity is up to 80%. The molecular weights of differential proteins are almost below 150-200 kDa. It is indicated that these small molecular proteins in these scope are potential biomarkers to cancer diagnosis. Moreover, CE-LIF could become a sensitive electrophoresis method for protein analysis for cancer diagnosis in the clinic study.

Biography :

Rong Wang has completed his Ph.D. at Lanzhou Institute of Chemical Physics, the Chinese Academy of Sciences and postdoctoral studies from Institute of Pharmacology and Toxicology, Academy of Military Medical Science. He is the Director of Master at Lanzhou University. He has finished the study on cancer diagnosis in the clinic. He is good at the analysis chemistry and biology, and bio-chromatography analysis.