Journal of Antivirals & Antiretrovirals
Open Access

Improvement of the PapMV nanoparticle adjuvant property through an increase of its avidity for the antigen (Infl uenza NP)

International Conference and Exhibition on VIROLOGY

5-7 September 2011 Baltimore, USA

Marie-Eve Laliberte Gagne

Posters: JAA

Abstract :

The principal caveat of existing infl uenza vaccine is their failure to provide long-term protection. Th is lack of effi ciency is caused by persistent (drift ) and dramatic (shift ) antigenic changes on the major surface proteins, the main target of protective immunity generated by traditional vaccines. Alternatively, vaccination with most conserved protein, like the nucleoprotein (NP) can stimulates immunity against multiple serotypes and could potentially provide an extended protection. Th e NP antigen contains more than 90% protein sequence homology among infl uenza A isolates and it also contains dominant CTL targets epitopes that made this antigen an attractive target for developing universal vaccine. Like many subunit vaccine, NP protein is a week antigen and need the use of adjuvant to increase its immunogenicity. We have developed an innovative high avidity VLP (HAV) nanoparticle to improve its adjuvant property to the NP antigen. Th e nanoparticles are derived from Papaya mosaic virus capsid protein (PapMV CP) produced in a bacteria expression system. We generated the HAV by adding an affi nity peptide directed to the NP protein at the surface of the VLPs. Th e fusions of the affi nity peptide to PapMV VLPs increased the avidity of PapMV VLPs to NP protein. Th is modifi cation anhanced the humoral and the cellular response directed to NP. Moreover, the immunity generated by the HAV adjuvanted NP protein vaccine increased the protection of vaccinated mice to a challenge with infl uenza virus. Th e protection was dependant of the CD8+ T-cells and was characterized by accelerated virus elimination aft er the onset of infection, rapid recovery and increase of survival rates.

Biography :

Ms Lalibert� Gagn� has obtained her Master?s degree in plant biology from the Infectious Disease Research Center (IDRC) of Laval University in Denis Leclerc?s laboratory. She has participated to publication of more than 6 papers in reputed journals.