Journal of Bacteriology & Parasitology

Influence of gelatin thrombin matrix tissue sealant on bacterial colony formation and risk of pelvic infection

4^th International Congress on Bacteriology and Infectious Diseases

May 16-18, 2016 San Antonio, USA

Michael J Jarrett

University of Colorado, USA

Posters & Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Objective: Gelatin thrombin matrix tissue sealant (GTM) use was previously identified as an independent predictor of pelvic infection following hysterectomies. We aim to elucidate contributing factors by assessing influence of GTM on bacterial colony formation and characterizing bacteria present at the vaginal cuff. Methods: Escherichia coli were incubated in phosphate buffered saline (PBS) and pelvic washings with and without GTM to assess influence on colony formation. Pelvic washings of the vaginal cuff were collected from hysterectomies occurring June through October 2015. In vitro techniques, quantitative polymerase chain reaction (qPCR) assays for 16S rRNA and 16S amplicon sequencing were performed with washings to characterize bacteria at the vaginal cuff. Results: Mean bacterial colony formation in PBS following 20 hours incubation was greater for E. coli with GTM versus without (1.48�?107 CFU/ml vs. 9.95�?105 CFU/ml, p=0.001). Out of 61 pelvic washings samples, 3 were culture positive (�?�5000 CFU/ml) with Enterococcus faecalis. Conclusion: In vitro experiments conclude GTM supports colony formation of E. coli in PBS. Analysis of pelvic washings revealed presence of E. faecalis but results were inconclusive. Further studies are recommended.

Biography :

Michael Jarrett holds a Master of Science degree in Biomedical Sciences from Colorado State University. He is currently a fourth-year medical student at the University of Colorado School of Medicine and will be graduating this May of 2016 with a Doctor of Medicine degree. He will completing his internship and residency in General Surgery at the University of Colorado. Michael is interested in research and Academic surgery and will be completing a research fellowship during his residency training.

Email: Michael.Jarrett@ucdenver.edu