Investigation of novel biomarkers for Alzheimer's disease using lipid-coated nanoparticles

2^nd World Congress on Biomarkers & Clinical Research

12-14 September 2011 Baltimore, USA

Hitoshi Sohma, Mami Yamaguchi, Michitoshi Kimura, Shin-ichi Imai, Kayo Matsumoto, Norio Takei and Yasuo Kokai

Scientific Tracks Abstracts: J Mol Biomark Diagn

Abstract :

Objective: Alzheimer�s disease (AD) diff ers from other forms of dementia in its relation to amyloid beta peptide (Abeta42). Abeta42, a proteolytic product of amyloid precursor proteins (APP), has a toxic eff ect on neuronal cells. Th is eff ect implies that protein expression is changed in neuronal cells by Abeta42, which provides a molecular marker for this disease. In the present study, we used the mice primary culture neurons and investigated the proteins in the supernatant aft er incubation with or without Abeta 42 . Methods: In view of the appearance of an acidic phospholipid (phosphatidylserine (PS)) on the outer plasma membrane of an apoptotic cell, we used PS as a probe and proteins bound to PS-coated magnetic nano-beads in a Ca 2+ -dependent manner were identifi ed using a proteomic approach. Results: Of a number of proteins identifi ed, we focused on annexin A5 and milk fat globule- EGF-factor 8 (MFG-E8) that is involved in the clearance of apoptotic cells. Both annexin A5 and MFG-E8 were found to be increased signifi cantly in the culture supernatant by Abeta42. Tg2576 mice (AD mouse model), which overexpress mutant human APP, showed signifi cant increase of annexin A5 in both the brain cortex and plasma, compared with control. Th e level of annexin A5 signifi cantly increased in a greater proportion of AD patients as compared to that in a control group ( p -value of less than 0.0001 in the logistic regression analysis). Conclusions: Both annexin A5 and MFG-E8 are novel plasma biomarker candidates for AD

Biography :

Hitoshi Sohma completed his Ph.D. in biochemistry at Hokkaido University, Japan, focusing on Ca2+-signaling in cell-cell communications, and his postdoctoral studies at the National Institute of Mental Health, NIH. He is a professor in the Department of Educational Development, Sapporo Medical University Center for Medical Education, Sapporo, Japan. He is now involved in both pathobiochemical research and the management of medical education at the university. He has published more than 50 papers in the bio-medical field.