Journal of Proteomics & Bioinformatics
Open Access

Mapping proteome alterations induced upon inflammatory activation, proliferation, and transformation to the nuclear extract, cytoplasm and secretome

2^nd International Conference on Proteomics & Bioinformatics

July 2-4, 2012 Embassy Suites Las Vegas, USA

Christopher Gerner

Scientific Tracks Abstracts: J Proteomics Bioinform

Abstract :

Proteome alterations observed upon functional activation or accompanying disease states of human cells may identify marker proteins which may qualify as biomarkers. In order to record characteristic features we analyzed cultured and primary human cells including leukocytes, fibroblasts, endothelial cells and epithelial cells at distinct functional states. Cells were characterized by FACS analysis and processed according to standard operation procedures to obtain nuclear, cytoplasmic and secreted protein fractions. Each protein fraction was fractionated by SDS-PAGE and gel slices were digested with trypsin. Peptides were separated applying CHIP-HPLC and analyzed by peptide fragmentation using an iontrap mass spectrometer. Data were collected and analyzed using the Griss Proteomics Database Engine. We observed that short term alterations of functional states such as inflammatory activation are accompanied by most significant alterations in the secretome, while long term alterations such as proliferation states are most clearly detected in the nuclear extract. Cytoplasmic proteins are best suitable to assess the cell type and the metabolic state. These systematic analyses shall support semi-automated data interpretation algorithms suitable for cells and tissue samples.

Biography :

Christopher Gerner has completed his Ph.D in 1998 from the University of Vienna and postdoctoral studies from Trinity College in Dublin. From 2002-2012 he was head of the Clinical Proteomics Laboratories at the Medical University of Vienna. Since 2012 he is full professor for bioanalysis at the University of Vienna.