Journal of Clinical and Cellular Immunology
Open Access

Molecular impact induced by different shaped graphene oxide on immune cells

4^th International Conference and Exhibition on Immunology

September 28-30, 2015 Crowne Plaza Houston River Oaks, Houston, TX, USA

Lucia Gemma Delogu1, Marco Orecchioni1, Dhifaf Jasim2, Mario Pescatori1, Francesco Sgarrella1, Davide Bedognetti3, Alberto Bianco4 and Kostas Kostarelos2

1University of Sassari, Italy 2University of Manchester, UK 3Sidra Medical and Research Center, Qatar 4Institute de Biologie Mol�?©culaire et Cellulaire, France

Scientific Tracks Abstracts: J Clin Cell Immunol

Abstract :

Graphene oxide (GO) is gaining the interest of the scientific community for its revolutionary future applications i.e. for drug delivery. In this context, the possible immune cell impact of GO is a fundamental area of study for a translational application in medicine. We focused on the effects, on human lymphomonocytes (PBMCs) of two types of GOs, deeply characterized, which differed in lateral size dimension (GO-Small: 140 nm, and GO-Large 4 �?¼m). To clarify the immune impact of GOs we provided a wide range of assays looking at cells viability, cell activation, cytokines release and gene expression. We let in lights also the impact of GOs on immune response-related 84 genes. GOs didnâ�?�?t impact the cell viability. In particular, the GOSmall modulated 16 genes (FR>4) compared to only 5 of GO-Large, evidencing a clear lateral dimension-dependent impact on cell activation. We confirmed the size-related effect at the protein level by multiplex ELISA. These evidences were also confirmed by microarray analysis on T and monocytes cell lines. GO-Small impact the immune cell activation, underlined by the over expression of genes such as CXCL10 ligand pathway and CXCR3 receptor. Data also evidenced the GO-Small-induced metabolism modulation in both cell types. Our work represents a comprehensive characterization of different sized GOs on immune cells giving crucial information for the chemical and physical design of graphene for biomedical applications i.e. as a new possible drug delivery systems and nanoimmunotherapy tools.

Biography :

Lucia Gemma Delogu has completed her PhD from Sassari University and Post-doctoral studies from University of Southern California. She is Assistant Professor of Biochemistry at the University of Sassari, Italy. She has published more than 21 papers in reputed journals and 9 as first or Senior/Corresponding author.

Email: lgdelogu@uniss.it