Biochemistry & Pharmacology: Open Access
Open Access
ISSN: 2167-0501
+44-20-4587-4809
ISSN: 2167-0501
+44-20-4587-4809
Mohammad Hayatul Islam, Iffat Zareen Ahmad and Mohammad Tariq Salman
Accepted Abstracts: Biochem & Pharmacol
Nigella sativa Linn. have many acclaimed medicinal properties, is an indigenous herbaceous plant belonging to the Ranunculaceae family. This study was designed to investigate the effects of N. sativa extract from ungerminative seed and from different germination phases of seed on the CNS responses in experimental animals. Anxiolytic, locomotor activity of extracts (1 g/kg) was evaluated in both stressed and unstressed animal model and antiepileptic effect was evaluated by maximal electroshock seizure model keeping diazepam (20 mg/kg) as a positive control. Antidepressant effect was evaluated by forced swim test and tail suspension test keeping imipramine (15 mg/kg) as a positive control. All tested extracts of N. sativa during different phases of germination showed significant anxiolytic effect in comparison to control (P<0.001). Diazepam reduced locomotor activity in unstressed rats did not affect in stressed rats while N. sativa extracts from germination phases significantly (P<0.001) reduced locomotor activity in unstressed as well as stressed animals. All the extracts of N. sativa from different germination phases exhibited significant (P<0.001) reduction in various phases of epileptic seizure on comparison with the reference standard diazepam. A significant reduction in the time required for the recovery during epilepsy was observed in 5th day germination extract treated groups. During antidepressant test N. sativa extracts did not exhibited significant reduction in immobility of rats. On the basis of all these results it was concluded that during germination especially in 5th day germination N. sativa have significant CNS depressant activity as compared to whole seed in may be due higher content of secondary metabolites during germination.
Mohammad Hayatul Islam is pursuing Ph.D. from Integral University, Lucknow, India. He has published a book and many research papers in reputed international journals. He has been awarded with national level fellowship during Ph.D. His expertise is in areas related to animal tissue culture and pharmacology.