Preparation of Au/silica/cellulose nanoparticle colloid solution and its use in X-ray imaging process
7th World Nano Conference
June 20-21, 2016 Cape Town, South Africa

Yoshio Kobayashi, Ryoko Nagasu, Tomohiko Nakagawa, Yohsuke Kubota, Kohsuke Gonda and Noriaki Ohuchi

Ibaraki University, Japan
Tohoku University, Japan

Scientific Tracks Abstracts: J Nanomed Nanotechnol

Abstract:

X-ray imaging of mice using a colloid solution of Au nanoparticles that were coated with silica and subsequently surface-modified with carboxymethylcellulose (CMC) (Au/SiO2/CMC) was performed in this work. The silica-coating for Au nanoparticles and the amination for silica-coated particles were simultaneously performed in the presence of the Au nanoparticles with a size of 17.9 nm, which were prepared by reducing Au ions (III) with sodium citrate in water at 80°C and by surface-modifying the Au nanoparticles with (3-aminopropyl)-trimethoxysilane, by a sol-gel process using tetraethylorthosilicate, (3-aminopropyl)-triethoxysilane, water and sodium hydroxide (Au/SiO2-NH2). The surface modification of Au/SiO2-NH2 particles with CMC was performed by simply adding CMC with carboxyl groups that react with an amino group to the Au/SiO2-NH2 particle colloid solution. The as-prepared the Au/SiO2/CMC particle colloid solution was concentrated by centrifugation for measurements using computed tomography (CT). Most particles contained a single core of the Au nanoparticles. Their particle size was 67.4±5.4 nm. A CT value of the Au/SiO2/CMC particle colloid solution with a Au concentration of 0.043 M was as high as 344±12 Hounsfield units (HU). This value corresponded to 8.0�?103 HU/M with respect to the Au concentration, which was larger than that of Iopamiron 300, a commercial X-ray contrast agent. Mouse tissues were imaged following injection of the Au/SiO2/CMC particle colloid solution.

Biography :

Yoshio Kobayashi received his BS (1988), MS (1990), and PhD (1993) degrees from Tohoku University. He worked as a Research Associate at Tohoku University (1993- 1997), as a Postdoctoral Researcher at Colorado State University (1997-1998), as a Postdoctoral Researcher at Kansas State University (1998-1999), as a Visiting Researcher at Universidade de Vigo (1999-2001), as a Research Associate at Tohoku University (2001-2002), and as an Associate Professor at Tohoku University (2002- 2006). He is a Professor of Ibaraki University, and has published more than 150 papers in reputed journals.

Email: yoshio.kobayashi.yk@vc.ibaraki.ac.jp