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Study of cytotoxicity of silver nanoparticles in vitro | 50849
Journal of Clinical Toxicology

Journal of Clinical Toxicology
Open Access

ISSN: 2161-0495

+44 1478 350008

Study of cytotoxicity of silver nanoparticles in vitro


International Toxicology Summit & Expo

November 26-28, 2012 Hilton San Antonio Airport, USA

Katerina Tomankova, Ales Panacek, Svatopluk Binder and Hana Kolarova

Posters: J Clinic Toxicol

Abstract :

S ilver nanoparticles (AgNPs) are used in consumer housholds, medical products and wound dressings due to their antimicrobial properties. However, in addition to toxic effects on microbes, AgNPs can also induce cytotoxicity in mammalian cells. The aim of the work was early identification of preventable risk factors connected with the consumers ́ usage of products of everyday use intended for contact with human skin. The risk factors are represented by substances with irritation potential and subsequent possible sensitisation, resulting in negative impact on human health. The legislation for cosmetics, chemical substances and other products requires for hazard identification the application of alternative toxicological methods in vitro without the use of animals. For this reason we used a battery of alternative assays in vitro based on cell cultures and tissue models. AgNPs were prepared by the unique method developed by Department of Physical Chemistry. The most data on the toxicity of AgNPs have been generated using nanoparticles modified by detergents, which may alter their toxicities. We studied the NPs disperged in water and revealed their genuine toxicity. Toxic effect of AgNPs was identified by viability tests on cell line NIH3T3 (mouse fibroblast cell) and tissue model EpiDerm EPI200. AgNPs were also tested using other methods based on fluorimetry and fluorescence (ROS, comet assay, etc.). Histology analysis revealed AgNPs localization in tissue cut of skin models. The results revealed the LC50 of AgNPs in different sizes of nanoparticles and non-toxic effect to the tissue models

Biography :

Katerina Tomankova has completed her Ph.D at the age of 26 in specialization of Medical Biophysics. She is an Assistant Professor at Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Czech Republic. She has published about 15 papers in reputed journals. Her basic research areas are biophysics, photobiology and in vitro toxicology.

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