Chidananda Nagamangala Kanchiswamy
Research and Innovation Centre, Italy
Chidananda Nagamangala Kanchiswamy did a PhD at the University of Turin in Torino (Italy), He spent most of his PhD research at Max Planck Institute for chemical ecology Jena and Center for Ecological Research Institute, Kyoto University (Japan) He now works as a postdoc (Marie Curie COFUND postdoc incoming 2010) at Foundation Edmund Mach, San Michele all'Adige, Trento, Italy, investigating on two exciting projects, 1) microbial volatile organic compounds perception, signal transduction and their effect on model and crop plants and 2) Next generation genome editing tools for fruit crop improvements.
The availability of genome sequences of many crop plants accompanied with revolution of new genome editing tools provided a breakthrough in modulating novel traits with unprecedented control and accuracy. However, plasmid mediated delivery of these genome editing components into cells can results in uncontrolled, random integration of plasmid sequences, potential safety problems, possible GMO regulations and other social hurdles. Here, we would like to propose direct delivery of purified Cas9 protein with guide RNA into plant cells. This method has shown high efficiency, significantly reduced off-target effects, and immediate genome editing after delivery compared to plasmid mediated genome editing. Furthermore, Cas9 protein-guide RNA complexes will be decomposed immediately after editing the gene of interest and thus there is huge possibility to be considered as Non-GMO crop plants.