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Eman Alaaeldin Abdelfattah has graduated from Faculty of Science, Cairo University, Egypt. She is an instructor at Entomology Department, Faculty of Science, Cairo University, Egypt.


Oxidative stressors may cause oxidative stress by generation of reactive oxygen species including free radicals and non free radicals, and therefore enhance oxidative damage to macromolecules. The aim of this work is not only to measure of products of ROS-oxidized macromolecules, but also to determine activity of antioxidant enzymes that were measured using spectrophotometric method and alkaline comet assay. The results show that each three stressors that were injected separately lead to formation of lipid peroxidation, protein carbonylation, and DNA strand breaks. Antioxidant response mechanism of the cells, as indicated from antioxidant enzymes level, lead to elevation of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) when compared to control (pvalue<0.05).