Maha Mohssen Abdelfattah
Cairo University, Egypt
Dr. Maha Mohssen has completed her M.D from Kasr El Eini Cairo University, she is a lecturer and researcher at the Micro Biology department at the Research Institute of Ophthalmology. Moreover, Dr. Maha also works as the head of the Bacteriology Corneal Unit at the Research Institute of Ophthalmology.
Dr. Maha has published several papers in reputed journals, and is also a member in the Egyptian Microbiology and Immunology Medical Society. In addition to the Egyptian Ophthalmological Society
Aim: To determine the possibility of the development of dry eye disease (DED) as a result of persistent infection with C. trachomatis and U. urealyticum in patients’ conjunctiva.
Methods: This study included 58 patients age range 20-50 years, diagnosed with DED. Control subjects were 27 non-dry eye group (same age range). Conjunctival scrapings were collected for bacterial culture and for detection of C. trachomatis and U.urealyticum by Direct Fluorescent Antibody (DFA) and Polymerase chain reaction (PCR) methods.
Results: C. trachomatis was detected in 65.5% and 76% of DED patients by DFA and PCR methods, respectively. U.urealyticum was found in 44.8% of DED patients by PCR method. Both organisms were identified in 37.9% of DED patients. Control subjects had C. trachomatis (22%) by DFA assay versus 7% by PCR; while U. urealyticum was detected in 3.7% by PCR. Conjunctival culture revealed coagulase negative Staphylococcus (CONS) the most common organism followed by Staphylococcus aureus, whereas gram negative Moraxella spp. was frequently isolated.
Conclusion: Results indicate C.trachomatis and U.urealyticum were detected in DED patients, and implicated in its development. Conjunctival microflora could show some potential value.
Keywords: Dry eye disease - conjunctiva - Chlamydia trachomatis - Ureaplasma urealyticum.
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