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Zhonglin Tang

Zhonglin Tang

Chinese Academy of Agricultural Sciences, China

Title: Systematic identification and characteristics of long non-coding RNAs in pigs

Biography

Zhonglin Tang has completed his PhD from Huazhong Agricultural University and Postdoctoral studies from Institute of Animal Science, Chinese Academy of Agricultural Science (CAAS). He is the PI of Agricultural Genome Institute at Shenzhen, CAAS. His field of interest is functional genomics and expression regulation of skeletal muscle in pig and mouse. He has published more than 44 papers in reputed journals and has been serving as an Editorial Board Member of repute.

Abstract

The long non-coding RNAs (lncRNAs) are non-protein coding RNAs and involve in a variety of biological processes. Pig (Susscrofa) is an important farm animal and ideal biomedical model. In this study, we performed a genome-wide scan of lncRNAs in pig. Based on strand-specific RNA-Seq data, a computational method was used to identify 96,314 novel lncRNAs candidates in pig genome which included 39,528 large intergenic ncRNAs (lincRNAs), 180 nature antisense transcripts and 56,606 intronic ncRNAs. Novel lncRNAs show fewer exon number, shorter transcripts length, lower GC content, expression and conservation level, higher SNP density than protein-coding transcripts. To validate the result, we perform RT-PCR to detect selected lncRNAs. Expression analysis of 5 abundant lncRNAs was conducted by quantitative PCR (qPCR) in 9 tissues of Landrace pigs in 160 days post-coitus (dpc). We first performed identification and characterization of pig lncRNAs using strand specific RNA sequencing. Our study significantly expanded the number of pig lncRNAs in non-protein coding RNA database and provided important information for functional studies.