Enzyme
Specificity
Cut positions (blunt - 5' ext. - 3' ext.)
AciI
CCGC
*136/138
BanII
GRGCYC
82/78
BsmAI
GTCTCNNNNN
29/33
Bsp1286I
GDGCHC
BsrDI
GCAATGNN
#127/125
DdeI
CTNAG
116/119
EcoP15I
CAGCAG(N)25NN
138/140
HgaI
GACGC(N)5(N)5
*44/49
HhaI
GCGC
*97/95
HinP1I
*95/97
Hpy188III
TCNNGA
71/73
MboII
GAAGA(N)7N
120/119
MwoI
GCNNNNNNNGC
86/83
SfcI
CTRYAG
90/94
TaqI
TCGA
42/44
Tsp509I
AATT
58/62