Enzyme

Specificity

Cut positions
(blunt - 5' ext. - 3' ext.)

AciI

CCGC

*136/138

BanII

GRGCYC

82/78

BsmAI

GTCTCNNNNN

29/33

Bsp1286I

GDGCHC

82/78

BsrDI

GCAATGNN

#127/125

DdeI

CTNAG

116/119

EcoP15I

CAGCAG(N)25NN

138/140

HgaI

GACGC(N)5(N)5

*44/49

HhaI

GCGC

*97/95

HinP1I

GCGC

*95/97

Hpy188III

TCNNGA

71/73

MboII

GAAGA(N)7N

120/119

MwoI

GCNNNNNNNGC

86/83

SfcI

CTRYAG

90/94

TaqI

TCGA

42/44

Tsp509I

AATT

58/62

Table 4: List of various one cutter restriction enzymes, their position and recognition site extracted using online tool NEBcutter, in partial nucleotide sequence of the test isolate of ZYMV.