Enzyme

Specificity

Cut positions
(blunt - 5' ext. - 3' ext.)

AluI

AGCT

89, 113

ApeKI

GCWGC

107/110, 110/113

BbvI

GCAGC(N)8NNNN

119/123, 122/126

Fnu4HI

GCNGC

108/109, 111/112

TseI

GCWGC

107/110, 110/113

Table 5: List of various two cutter restriction enzymes, their position and recognition site extracted using online tool NEBcutter, in partial nucleotide sequence of the test isolate of ZYMV.