Figure 5: Immunohistochemical labeling of SERPINA3. A: Whole mount preparation of frontal cortex from a control showing low diffuse labeling of the cortical ribbon and low intensity staining of white matter (w). (SERPINA3, Obj x 5). B: Whole mount preparation of frontal cortex from a case of TBM showing diffuse intense staining of the cortical ribbon and exudates in the subarachnoid space (w - white matter). (SERPINA3, Obj x 5). C: The pyramidal neurons of the superficial cortex in controls have cytoplasmic labeling. (SERPINA3, Obj x 20). D: The astrocytes in the cortex of controls are stained well, though the density of labeled astrocytes is low. (SERPINA3, Obj x 20). E: The white matter shows a higher density of the SERPINA3 labeled astrocytes in TBM. (SERPINA3, Obj x 10). F: Low power view of the frontal cortex showing dense labeling of subpial glial membrane, reactive astrocytes underneath and the granulomatous exudates in the subarachnoid space. (TBM case) (v: vessel) (SERPINA3, Obj x 20). G: Higher magnification of the subarachnoid exudates showing labeling of the round histocytes. (TBM case) (SERPINA3, Obj x 20).