Figure 3: 2-DE of secreted proteins. (A) The secreted proteins extracted by Ammonium sulfate precipitation. (B) The secreted proteins extracted by modified sevage method. The protein samples from both methods were performed on IPG trips with pH range 3-10 for first dimension and 12.5% polyacrylamide gels for second dimension. The proteins were visualized using sliver staining. Protein spots which were marked by arrows were chosen for mass spectrum analysis and identified as 22kDa glycoprotein (Cryphonectria parasitica database accession number 100735).